2bod: Difference between revisions
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[[Image:2bod.gif|left|200px]] | [[Image:2bod.gif|left|200px]] | ||
'''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE''' | {{Structure | ||
|PDB= 2bod |SIZE=350|CAPTION= <scene name='initialview01'>2bod</scene>, resolution 1.50Å | |||
|SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+For+Chain+X'>AC1</scene> | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] | |||
|GENE= | |||
}} | |||
'''CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2BOD is a [ | 2BOD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOD OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:[http:// | Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16185060 16185060] | ||
[[Category: Cellulase]] | [[Category: Cellulase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tim a/b fold]] | [[Category: tim a/b fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:04:35 2008'' | ||
Revision as of 14:04, 20 March 2008
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| 2bod, resolution 1.50Å | |||||||||||||
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| Sites: | AC1 | ||||||||||||
| Activity: | Cellulase, with EC number 3.2.1.4 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A FROM THERMOBIFIDA FUSCA IN COMPLEX WITH METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE
Overview
Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution.
About this Structure
2BOD is a Single protein structure of sequence from Thermobifida fusca. Full crystallographic information is available from OCA.
Reference
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion., Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA, Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060
Page seeded by OCA on Thu Mar 20 16:04:35 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Cellulase
- Single protein
- Thermobifida fusca
- Bergfors, T.
- Driguez, H.
- Dultz, E.
- Irwin, D C.
- Jones, T A.
- Larsson, A M.
- Roos, A.
- Wilson, D B.
- Carbohydrate metabolism
- Cellulose degradation
- Endoglucanase
- Glycosidase
- Glycoside hydrolase family 6
- Hydrolase
- Methyl cellobiosyl-4-thio-beta-cellobioside
- Polysaccharide degradation
- Tim a/b fold
