1w36: Difference between revisions
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==Overview== | ==Overview== | ||
RecBCD is a multi-functional enzyme complex that processes DNA ends, resulting from a double-strand break. RecBCD is a bipolar helicase that, splits the duplex into its component strands and digests them until, encountering a recombinational hotspot (Chi site). The nuclease activity, is then attenuated and RecBCD loads RecA onto the 3' tail of the DNA. Here, we present the crystal structure of RecBCD bound to a DNA substrate. In, this initiation complex, the DNA duplex has been split across the RecC, subunit to create a fork with the separated strands each heading towards, different helicase motor subunits. The strands pass along tunnels within, the complex, both emerging adjacent to the nuclease domain of RecB., Passage of the 3' tail through one of these tunnels provides a mechanism, .. | RecBCD is a multi-functional enzyme complex that processes DNA ends, resulting from a double-strand break. RecBCD is a bipolar helicase that, splits the duplex into its component strands and digests them until, encountering a recombinational hotspot (Chi site). The nuclease activity, is then attenuated and RecBCD loads RecA onto the 3' tail of the DNA. Here, we present the crystal structure of RecBCD bound to a DNA substrate. In, this initiation complex, the DNA duplex has been split across the RecC, subunit to create a fork with the separated strands each heading towards, different helicase motor subunits. The strands pass along tunnels within, the complex, both emerging adjacent to the nuclease domain of RecB., Passage of the 3' tail through one of these tunnels provides a mechanism, for the recognition of a Chi sequence by RecC within the context of, double-stranded DNA. Gating of this tunnel suggests how nuclease activity, might be regulated. | ||
==About this Structure== | ==About this Structure== | ||
1W36 is a | 1W36 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_V Exodeoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.5 3.1.11.5] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W36 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:26:42 2007'' | ||
