2cfa: Difference between revisions
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[[Image:2cfa.gif|left|200px]] | [[Image:2cfa.gif|left|200px]] | ||
'''STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX''' | {{Structure | ||
|PDB= 2cfa |SIZE=350|CAPTION= <scene name='initialview01'>2cfa</scene>, resolution 2.30Å | |||
|SITE= <scene name='pdbsite=AC1:Fad+Binding+Site+For+Chain+B'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2CFA is a [ | 2CFA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. | ||
==Reference== | ==Reference== | ||
Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX., Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U, J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:[http:// | Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX., Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U, J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16707489 16707489] | ||
[[Category: Paramecium bursaria chlorella virus 1]] | [[Category: Paramecium bursaria chlorella virus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: CME]] | [[Category: CME]] | ||
[[Category: FAD]] | [[Category: FAD]] | ||
[[Category: | [[Category: fdt]] | ||
[[Category: flavin dependent thymidylate synthase fad]] | [[Category: flavin dependent thymidylate synthase fad]] | ||
[[Category: flavoprotein]] | [[Category: flavoprotein]] | ||
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[[Category: tscp]] | [[Category: tscp]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:14:37 2008'' | ||
Revision as of 14:14, 20 March 2008
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| 2cfa, resolution 2.30Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sites: | AC1 | ||||||||||||
| Ligands: | CME and FAD | ||||||||||||
| Activity: | Thymidylate synthase (FAD), with EC number 2.1.1.148 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX
Overview
By using biochemical and structural analyses, we have investigated the catalytic mechanism of the recently discovered flavin-dependent thymidylate synthase ThyX from Paramecium bursaria chlorella virus-1 (PBCV-1). Site-directed mutagenesis experiments have identified several residues implicated in either NADPH oxidation or deprotonation activity of PBCV-1 ThyX. Chemical modification by diethyl pyrocarbonate and mass spectroscopic analyses identified a histidine residue (His53) crucial for NADPH oxidation and located in the vicinity of the redox active N-5 atom of the FAD ring system. Moreover, we observed that the conformation of active site key residues of PBCV-1 ThyX differs from earlier reported ThyX structures, suggesting structural changes during catalysis. Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate.
About this Structure
2CFA is a Protein complex structure of sequences from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX., Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U, J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:16707489
Page seeded by OCA on Thu Mar 20 16:14:37 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Paramecium bursaria chlorella virus 1
- Protein complex
- Thymidylate synthase (FAD)
- Bernauer, J.
- Decottignies, P.
- Graille, M.
- Graziani, S.
- Liebl, U.
- Marchand, C.
- Myllykallio, H.
- Skouloubris, S.
- Tilbeurgh, H Van.
- Zhou, C Z.
- CME
- FAD
- Fdt
- Flavin dependent thymidylate synthase fad
- Flavoprotein
- Methyltransferase
- Nucleotide biosynthesis
- Paramecium bursaria chlorella virus-1
- Thyx
- Transferase
- Tscp
