2cme: Difference between revisions
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[[Image:2cme.gif|left|200px]] | [[Image:2cme.gif|left|200px]] | ||
'''THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN''' | {{Structure | ||
|PDB= 2cme |SIZE=350|CAPTION= <scene name='initialview01'>2cme</scene>, resolution 2.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=D10:DECANE'>D10</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2CME is a [ | 2CME is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CME OCA]. | ||
==Reference== | ==Reference== | ||
The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus., Meier C, Aricescu AR, Assenberg R, Aplin RT, Gilbert RJ, Grimes JM, Stuart DI, Structure. 2006 Jul;14(7):1157-65. PMID:[http:// | The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus., Meier C, Aricescu AR, Assenberg R, Aplin RT, Gilbert RJ, Grimes JM, Stuart DI, Structure. 2006 Jul;14(7):1157-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16843897 16843897] | ||
[[Category: Human sars coronavirus]] | [[Category: Human sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: virus assembly]] | [[Category: virus assembly]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:17:14 2008'' | ||
Revision as of 14:17, 20 March 2008
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| 2cme, resolution 2.8Å | |||||||||||||
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| Ligands: | D10 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
THE CRYSTAL STRUCTURE OF SARS CORONAVIRUS ORF-9B PROTEIN
Overview
To achieve the greatest output from their limited genomes, viruses frequently make use of alternative open reading frames, in which translation is initiated from a start codon within an existing gene and, being out of frame, gives rise to a distinct protein product. These alternative protein products are, as yet, poorly characterized structurally. Here we report the crystal structure of ORF-9b, an alternative open reading frame within the nucleocapsid (N) gene from the SARS coronavirus. The protein has a novel fold, a dimeric tent-like beta structure with an amphipathic surface, and a central hydrophobic cavity that binds lipid molecules. This cavity is likely to be involved in membrane attachment and, in mammalian cells, ORF-9b associates with intracellular vesicles, consistent with a role in the assembly of the virion. Analysis of ORF-9b and other overlapping genes suggests that they provide snapshots of the early evolution of novel protein folds.
About this Structure
2CME is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.
Reference
The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus., Meier C, Aricescu AR, Assenberg R, Aplin RT, Gilbert RJ, Grimes JM, Stuart DI, Structure. 2006 Jul;14(7):1157-65. PMID:16843897
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