2cxa: Difference between revisions
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[[Image:2cxa.gif|left|200px]] | [[Image:2cxa.gif|left|200px]] | ||
'''Crystal structure of Leucyl/phenylalanyl-tRNA protein transferase from Escherichia coli''' | {{Structure | ||
|PDB= 2cxa |SIZE=350|CAPTION= <scene name='initialview01'>2cxa</scene>, resolution 1.60Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Leucyltransferase Leucyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.6 2.3.2.6] | |||
|GENE= | |||
}} | |||
'''Crystal structure of Leucyl/phenylalanyl-tRNA protein transferase from Escherichia coli''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2CXA is a [ | 2CXA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXA OCA]. | ||
==Reference== | ==Reference== | ||
The crystal structure of leucyl/phenylalanyl-tRNA-protein transferase from Escherichia coli., Dong X, Kato-Murayama M, Muramatsu T, Mori H, Shirouzu M, Bessho Y, Yokoyama S, Protein Sci. 2007 Mar;16(3):528-34. Epub 2007 Jan 22. PMID:[http:// | The crystal structure of leucyl/phenylalanyl-tRNA-protein transferase from Escherichia coli., Dong X, Kato-Murayama M, Muramatsu T, Mori H, Shirouzu M, Bessho Y, Yokoyama S, Protein Sci. 2007 Mar;16(3):528-34. Epub 2007 Jan 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17242373 17242373] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Leucyltransferase]] | [[Category: Leucyltransferase]] | ||
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[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
[[Category: aminoacyl-trna]] | [[Category: aminoacyl-trna]] | ||
[[Category: national project on protein structural and functional | [[Category: national project on protein structural and functional analyse]] | ||
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: protein degradation]] | [[Category: protein degradation]] | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:20:53 2008'' | ||
Revision as of 14:20, 20 March 2008
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| 2cxa, resolution 1.60Å | |||||||||||||
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| Activity: | Leucyltransferase, with EC number 2.3.2.6 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of Leucyl/phenylalanyl-tRNA protein transferase from Escherichia coli
Overview
Leucyl/phenylalanyl-tRNA-protein transferase (L/F-transferase) is an N-end rule pathway enzyme, which catalyzes the transfer of Leu and Phe from aminoacyl-tRNAs to exposed N-terminal Arg or Lys residues of acceptor proteins. Here, we report the 1.6 A resolution crystal structure of L/F-transferase (JW0868) from Escherichia coli, the first three-dimensional structure of an L/F-transferase. The L/F-transferase adopts a monomeric structure consisting of two domains that form a bilobate molecule. The N-terminal domain forms a small lobe with a novel fold. The large C-terminal domain has a highly conserved fold, which is observed in the GCN5-related N-acetyltransferase (GNAT) family. Most of the conserved residues of L/F-transferase reside in the central cavity, which exists at the interface between the N-terminal and C-terminal domains. A comparison of the structures of L/F-transferase and the bacterial peptidoglycan synthase FemX, indicated a structural homology in the C-terminal domain, and a similar domain interface region. Although the peptidyltransferase function is shared between the two proteins, the enzymatic mechanism would differ. The conserved residues in the central cavity of L/F-transferase suggest that this region is important for the enzyme catalysis.
About this Structure
2CXA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The crystal structure of leucyl/phenylalanyl-tRNA-protein transferase from Escherichia coli., Dong X, Kato-Murayama M, Muramatsu T, Mori H, Shirouzu M, Bessho Y, Yokoyama S, Protein Sci. 2007 Mar;16(3):528-34. Epub 2007 Jan 22. PMID:17242373
Page seeded by OCA on Thu Mar 20 16:20:53 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Escherichia coli
- Leucyltransferase
- Single protein
- Bessho, Y.
- Kato-Murayama, M.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Shirouzu, M.
- Yokoyama, S.
- Aminoacyl-trna
- National project on protein structural and functional analyse
- Nppsfa
- Protein degradation
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomic
- Transferase
