2czt: Difference between revisions
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[[Image:2czt.gif|left|200px]] | [[Image:2czt.gif|left|200px]] | ||
'''lipocalin-type prostaglandin D synthase''' | {{Structure | ||
|PDB= 2czt |SIZE=350|CAPTION= <scene name='initialview01'>2czt</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] | |||
|GENE= | |||
}} | |||
'''lipocalin-type prostaglandin D synthase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2CZT is a [ | 2CZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZT OCA]. | ||
==Reference== | ==Reference== | ||
Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:[http:// | Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12761195 12761195] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Prostaglandin-D synthase]] | [[Category: Prostaglandin-D synthase]] | ||
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[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:21:37 2008'' | ||
Revision as of 14:21, 20 March 2008
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| 2czt, resolution 2.00Å | |||||||||||||
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| Activity: | Prostaglandin-D synthase, with EC number 5.3.99.2 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
lipocalin-type prostaglandin D synthase
Overview
Lipocalin-type prostaglandin D synthase is the key enzyme for the production of prostaglandin D(2), a potent endogenous somnogen, in the brain. We cloned, produced, and crystallized the native enzyme and selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the hanging drop vapor-diffusion method with both malonate and citrate as precipitants. The native crystals obtained with malonate belong to orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with citrate belong to orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond 2.1 A resolution.
About this Structure
2CZT is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:12761195
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Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Mus musculus
- Prostaglandin-D synthase
- Single protein
- Ago, H.
- Aritake, K.
- Hayaishi, O.
- Inoue, T.
- Irikura, D.
- Kumasaka, T.
- Miyano, M.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Urade, Y.
- Yamamoto, M.
- C2221 native
- Lipocalin
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomic
