1e9m: Difference between revisions
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==Overview== | ==Overview== | ||
A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter, capsulatus has been purified in recombinant form from Escherichia coli., This protein, called FdVI, resembles ferredoxins involved in iron-sulfur, cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified, recombinant FdVI was recovered in high yields and appeared to be, indistinguishable from the genuine R. capsulatus ferredoxin based on, UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has, been crystallized in the oxidized state by a sitting-drop vapour-diffusion, technique using sodium formate as precipitant. Seeding larger drops from a, previous hanging-drop-grown small crystal resulted in the formation of, long red-brown prismatic needles. Preliminary X-ray diffraction ... | A [2Fe-2S] ferredoxin found in the photosynthetic bacterium Rhodobacter, capsulatus has been purified in recombinant form from Escherichia coli., This protein, called FdVI, resembles ferredoxins involved in iron-sulfur, cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified, recombinant FdVI was recovered in high yields and appeared to be, indistinguishable from the genuine R. capsulatus ferredoxin based on, UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has, been crystallized in the oxidized state by a sitting-drop vapour-diffusion, technique using sodium formate as precipitant. Seeding larger drops from a, previous hanging-drop-grown small crystal resulted in the formation of, long red-brown prismatic needles. Preliminary X-ray diffraction analysis, indicated that FdVI crystals are orthorhombic and belong to the space, group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c =, 54.29 A. | ||
==About this Structure== | ==About this Structure== | ||
1E9M is a | 1E9M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus] with FES as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: FES. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E9M OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ferredoxin]] | [[Category: ferredoxin]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:32:12 2007'' | ||
