4ksr

Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer

spinqualitylabelsall models 4ksr, resolution 4.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ksr is a 3 chain structure with sequence from Pseab. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 4kss Gene: epsE, VC_2732, PA14_01030 (PSEAB) Resources: FirstGlance, OCA, RCSB, PDBsum Function [GSPE_VIBCH] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane. Publication Abstract from PubMed The type II secretion system (T2SS), a multiprotein machinery spanning two membranes in Gram-negative bacteria, is responsible for the secretion of folded proteins from the periplasm across the outer membrane. The critical multidomain T2SS assembly ATPase GspEEpsE had not been structurally characterized as a hexamer. Here, four hexamers of Vibrio cholerae GspEEpsE are obtained when fused to Hcp1 as an assistant hexamer, as shown with native mass spectrometry. The enzymatic activity of the GspEEpsE-Hcp1 fusions is approximately 20 times higher than that of a GspEEpsE monomer, indicating that increasing the local concentration of GspEEpsE by the fusion strategy was successful. Crystal structures of GspEEpsE-Hcp1 fusions with different linker lengths reveal regular and elongated hexamers of GspEEpsE with major differences in domain orientation within subunits, and in subunit assembly. SAXS studies on GspEEpsE-Hcp1 fusions suggest that even further variability in GspEEpsE hexamer architecture is likely. Hexamers of the Type II Secretion ATPase GspE from Vibrio cholerae with Increased ATPase Activity.,Lu C, Turley S, Marionni ST, Park YJ, Lee KK, Patrick M, Shah R, Sandkvist M, Bush MF, Hol WG Structure. 2013 Aug 13. pii: S0969-2126(13)00256-6. doi:, 10.1016/j.str.2013.06.027. PMID:23954505 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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