1uoc: Difference between revisions
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==Overview== | ==Overview== | ||
In Saccharomyces cerevisiae, a large complex, known as the Ccr4-Not, complex, containing two nucleases, is responsible for mRNA deadenylation., One of these nucleases is called Pop2 and has been identified by, similarity with PARN, a human poly(A) nuclease. Here, we present the, crystal structure of the nuclease domain of Pop2 at 2.3 A resolution. The, domain has the fold of the DnaQ family and represents the first structure, of an RNase from the DEDD superfamily. Despite the presence of two, non-canonical residues in the active site, the domain displays RNase, activity on a broad range of RNA substrates. Site-directed mutagenesis of, active-site residues demonstrates the intrinsic ability of the Pop2 RNase, D domain to digest RNA. This first structure of a nuclease involved in the, 3'-5' . | In Saccharomyces cerevisiae, a large complex, known as the Ccr4-Not, complex, containing two nucleases, is responsible for mRNA deadenylation., One of these nucleases is called Pop2 and has been identified by, similarity with PARN, a human poly(A) nuclease. Here, we present the, crystal structure of the nuclease domain of Pop2 at 2.3 A resolution. The, domain has the fold of the DnaQ family and represents the first structure, of an RNase from the DEDD superfamily. Despite the presence of two, non-canonical residues in the active site, the domain displays RNase, activity on a broad range of RNA substrates. Site-directed mutagenesis of, active-site residues demonstrates the intrinsic ability of the Pop2 RNase, D domain to digest RNA. This first structure of a nuclease involved in the, 3'-5' deadenylation of mRNA in yeast provides information for the, understanding of the mechanism by which the Ccr4-Not complex achieves its, functions. | ||
==About this Structure== | ==About this Structure== | ||
1UOC is a | 1UOC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA and XE as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UOC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:33:43 2007'' | ||
