2de6: Difference between revisions
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'''The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase''' | {{Structure | ||
|PDB= 2de6 |SIZE=350|CAPTION= <scene name='initialview01'>2de6</scene>, resolution 1.80Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> and <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2DE6 is a [ | 2DE6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Janthinobacterium Janthinobacterium] and [http://en.wikipedia.org/wiki/Pseudomonas_resinovorans Pseudomonas resinovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DE6 OCA]. | ||
==Reference== | ==Reference== | ||
Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system., Ashikawa Y, Fujimoto Z, Noguchi H, Habe H, Omori T, Yamane H, Nojiri H, Structure. 2006 Dec;14(12):1779-89. PMID:[http:// | Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system., Ashikawa Y, Fujimoto Z, Noguchi H, Habe H, Omori T, Yamane H, Nojiri H, Structure. 2006 Dec;14(12):1779-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17161368 17161368] | ||
[[Category: Janthinobacterium]] | [[Category: Janthinobacterium]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: terminal oxygenase]] | [[Category: terminal oxygenase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:26:12 2008'' | ||
Revision as of 14:26, 20 March 2008
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| 2de6, resolution 1.80Å | |||||||||||||
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| Ligands: | FE2 and FES | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
The reduced complex between oxygenase and ferredoxin in carbazole 1,9a-dioxygenase
Overview
Carbazole 1,9a-dioxygenase (CARDO), a member of the Rieske nonheme iron oxygenase system (ROS), consists of a terminal oxygenase (CARDO-O) and electron transfer components (ferredoxin [CARDO-F] and ferredoxin reductase [CARDO-R]). We determined the crystal structures of the nonreduced, reduced, and substrate-bound binary complexes of CARDO-O with its electron donor, CARDO-F, at 1.9, 1.8, and 2.0 A resolutions, respectively. These structures provide the first structure-based interpretation of intercomponent electron transfer between two Rieske [2Fe-2S] clusters of ferredoxin and oxygenase in ROS. Three molecules of CARDO-F bind to the subunit boundary of one CARDO-O trimeric molecule, and specific binding created by electrostatic and hydrophobic interactions with conformational changes suitably aligns the two Rieske clusters for electron transfer. Additionally, conformational changes upon binding carbazole resulted in the closure of a lid over the substrate-binding pocket, thereby seemingly trapping carbazole at the substrate-binding site.
About this Structure
2DE6 is a Protein complex structure of sequences from Janthinobacterium and Pseudomonas resinovorans. Full crystallographic information is available from OCA.
Reference
Electron transfer complex formation between oxygenase and ferredoxin components in Rieske nonheme iron oxygenase system., Ashikawa Y, Fujimoto Z, Noguchi H, Habe H, Omori T, Yamane H, Nojiri H, Structure. 2006 Dec;14(12):1779-89. PMID:17161368
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