2dep: Difference between revisions
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[[Image:2dep.jpg|left|200px]] | [[Image:2dep.jpg|left|200px]] | ||
'''Crystal Structure of xylanase B from Clostridium stercorarium F9''' | {{Structure | ||
|PDB= 2dep |SIZE=350|CAPTION= <scene name='initialview01'>2dep</scene>, resolution 1.80Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= xynB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1510 Clostridium stercorarium]) | |||
}} | |||
'''Crystal Structure of xylanase B from Clostridium stercorarium F9''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2DEP is a [ | 2DEP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA]. | ||
==Reference== | ==Reference== | ||
Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:[http:// | Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747719 14747719] | ||
[[Category: Clostridium stercorarium]] | [[Category: Clostridium stercorarium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: family 10]] | [[Category: family 10]] | ||
[[Category: glycosidase]] | [[Category: glycosidase]] | ||
[[Category: national project on protein structural and functional | [[Category: national project on protein structural and functional analyse]] | ||
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: xylan degradation]] | [[Category: xylan degradation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:26:23 2008'' | ||
Revision as of 14:26, 20 March 2008
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| 2dep, resolution 1.80Å | |||||||||||||
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| Gene: | xynB (Clostridium stercorarium) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of xylanase B from Clostridium stercorarium F9
Overview
Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
About this Structure
2DEP is a Single protein structure of sequence from Clostridium stercorarium. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:14747719
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