3t72

PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex

spinqualitylabelsall models 3t72, resolution 4.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3t72 is a 50 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: phoB, b0399, JW0389 (ECOLI), rpoD, alt, b3067, JW3039, EKO11_4334, rpoB (ECOLI) Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6 Resources: FirstGlance, OCA, RCSB, PDBsum Function [RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium. [PHOB_ECOLI] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited. Publication Abstract from PubMed PhoB is a two-component response regulator that activates transcription by interacting with the sigma(70) subunit of the E. coli RNA polymerase in promoters in which the -35 sigma(70)-recognition element is replaced by the pho box. The crystal structure of a transcription initiation subcomplex that includes the sigma(4) domain of sigma(70) fused with the RNA polymerase beta subunit flap tip helix, the PhoB effector domain and the pho box DNA reveals how sigma(4) recognizes the upstream pho box repeat. As with the -35 element, sigma(4) achieves this recognition through the N-terminal portion of its DNA recognition helix, but contact with the DNA major groove is less extensive. Unexpectedly, the same recognition helix contacts the transactivation loop and helices alpha2 and alpha3 of PhoB. This result shows a simple and elegant mechanism for polymerase recruitment to pho box promoters in which the lost -35 element contacts are compensated by new ones with the activator. In addition, sigma(4) is reoriented, thereby suggesting a remodelling mechanism for transcription initiation. The structure of a transcription activation subcomplex reveals how sigma(70) is recruited to PhoB promoters.,Blanco AG, Canals A, Bernues J, Sola M, Coll M EMBO J. 2011 Aug 9;30(18):3776-85. doi: 10.1038/emboj.2011.271. PMID:21829166 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also RNA polymerase

Proteopedia Page Contributors and Editors (what is this?)

OCA