1h0d: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally . | The murine monoclonal antibody 26-2F neutralizes the angiogenic and, ribonucleolytic activities of human angiogenin (ANG) and is highly, effective in preventing the establishment and metastatic dissemination of, human tumors in athymic mice. Here we report a 2.0 A resolution crystal, structure for the complex of ANG with the Fab fragment of 26-2F that, reveals the detailed interactions between ANG and the, complementarity-determining regions (CDRs) of the antibody. Surprisingly, Fab binding induces a dramatic conformational change in the cell binding, region of ANG at the opposite end of the molecule from the combining site;, crosslinking experiments indicate that this rearrangement also occurs in, solution. The ANG-Fab complex structure should be invaluable for designing, maximally humanized versions of 26-2F for potential clinical use. | ||
==About this Structure== | ==About this Structure== | ||
1H0D is a | 1H0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: SA1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H0D OCA]. | ||
==Reference== | ==Reference== | ||
| Line 22: | Line 22: | ||
[[Category: ribonuclease]] | [[Category: ribonuclease]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:34:50 2007'' | ||
