2p1w

structure of the phosphothreonine lyase SpvC, the effector protein from Salmonella

spinqualitylabelsall models 2p1w, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2p1w is a 1 chain structure with sequence from Salmonella enterica subsp. enterica serovar enteritidis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: MSE Gene: SpvC (Salmonella enterica subsp. enterica serovar Enteritidis) Resources: FirstGlance, OCA, RCSB, PDBsum Function [VRP3_SALEN] Not known. This protein is involved in the virulence of salmonellas. Publication Abstract from PubMed The OspF family of phosphothreonine lyase, including SpvC from Salmonella, irreversibly inactivates the dual-phosphorylated host MAPKs (pT-X-pY) through beta elimination. We determined crystal structures of SpvC and its complex with a phosphopeptide substrate. SpvC adopts a unique fold of alpha/beta type. The disordered N terminus harbors a canonical D motif for MAPK substrate docking. The enzyme-substrate complex structure indicates that recognition of the phosphotyrosine followed by insertion of the threonine phosphate into an arginine pocket places the phosphothreonine into the enzyme active site. This requires the conformational flexibility of pT-X-pY, which suggests that p38 (pT-G-pY) is likely the preferred physiological substrate. Structure-based biochemical and enzymatic analysis allows us to propose a general acid/base mechanism for beta elimination reaction catalyzed by the phosphothreonine lyase. The mechanism described here provides a structural understanding of MAPK inactivation by a family of pathogenic effectors conserved in plant and animal systems and may also open a new route for biological catalysis. Structural insights into the enzymatic mechanism of the pathogenic MAPK phosphothreonine lyase.,Zhu Y, Li H, Long C, Hu L, Xu H, Liu L, Chen S, Wang DC, Shao F Mol Cell. 2007 Dec 14;28(5):899-913. Epub 2007 Nov 29. PMID:18060821 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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