2m8f: Difference between revisions

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{{STRUCTURE_2m8f| PDB=2m8f | SCENE= }}
==Structure of lasso peptide astexin3==
===Structure of lasso peptide astexin3===
<StructureSection load='2m8f' size='340' side='right' caption='[[2m8f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
{{ABSTRACT_PUBMED_23862624}}
== Structural highlights ==
<table><tr><td colspan='2'>[[2m8f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asticcacaulis_excentricus_cb_48 Asticcacaulis excentricus cb 48]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M8F FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Astex_2447 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573065 Asticcacaulis excentricus CB 48])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m8f RCSB], [http://www.ebi.ac.uk/pdbsum/2m8f PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lasso peptides are a class of ribosomally-derived natural products with diverse bioactivities. The characteristic threaded lasso structure in these peptides derives from an isopeptide bond attaching the N-terminus of the peptide to an acidic sidechain. Here we describe the heterologous expression of a lasso peptide gene cluster encoding two lasso peptides, astexin-2 and astexin-3 and solve the solution structure of astexin-3. This cluster also encodes an enzyme annotated as a protease. We show that this enzyme, AtxE2, is a lasso peptide isopeptidase that specifically hydrolyzes astexins-2 and -3 converting them to linear peptides. Astexin-3 is highly thermostable and resists unthreading after extensive heat treatment. In contrast, astexin-2 unthreads upon heat treatment. AtxE2 has no activity toward unthreaded astexin-2 demonstrating that this isopeptidase must recognize a knotted structure in order to function. We also use this isopeptidase as a tool to study evolutionary relationships between lasso peptide gene clusters.


==About this Structure==
Discovery and Characterization of an Isopeptidase that Linearizes Lasso Peptides.,Maksimov MO, Link AJ J Am Chem Soc. 2013 Jul 17. PMID:23862624<ref>PMID:23862624</ref>
[[2m8f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asticcacaulis_excentricus_cb_48 Asticcacaulis excentricus cb 48]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M8F OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023862624</ref><references group="xtra"/><references/>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Asticcacaulis excentricus cb 48]]
[[Category: Asticcacaulis excentricus cb 48]]
[[Category: Link, A.]]
[[Category: Link, A]]
[[Category: Maksimov, M O.]]
[[Category: Maksimov, M O]]
[[Category: Sidechain-to-backbone link]]
[[Category: Sidechain-to-backbone link]]
[[Category: Unknown function]]
[[Category: Unknown function]]
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