2eig: Difference between revisions
New page: left|200px<br /><applet load="2eig" size="350" color="white" frame="true" align="right" spinBox="true" caption="2eig, resolution 2.00Å" /> '''Lotus tetragonolobus... |
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[[Image:2eig.jpg|left|200px]] | [[Image:2eig.jpg|left|200px]] | ||
'''Lotus tetragonolobus seed lectin (Isoform)''' | {{Structure | ||
|PDB= 2eig |SIZE=350|CAPTION= <scene name='initialview01'>2eig</scene>, resolution 2.00Å | |||
|SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Nag+Binding+Site+For+Residue+B+3001'>AC2</scene>, <scene name='pdbsite=AC3:Mn+Binding+Site+For+Residue+A+1101'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Residue+A+1102'>AC4</scene>, <scene name='pdbsite=AC5:Mn+Binding+Site+For+Residue+B+1201'>AC5</scene>, <scene name='pdbsite=AC6:Ca+Binding+Site+For+Residue+B+1202'>AC6</scene>, <scene name='pdbsite=AC7:Mn+Binding+Site+For+Residue+C+1301'>AC7</scene>, <scene name='pdbsite=AC8:Ca+Binding+Site+For+Residue+C+1302'>AC8</scene>, <scene name='pdbsite=AC9:Mn+Binding+Site+For+Residue+D+1401'>AC9</scene> and <scene name='pdbsite=BC1:Ca+Binding+Site+For+Residue+D+1402'>BC1</scene> | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Lotus tetragonolobus seed lectin (Isoform)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2EIG is a [ | 2EIG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lotus_tetragonolobus Lotus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIG OCA]. | ||
==Reference== | ==Reference== | ||
Identification of a new quaternary association for legume lectins., Moreno FB, de Oliveira TM, Martil DE, Vicoti MM, Bezerra GA, Abrego JR, Cavada BS, Filgueira de Azevedo W Jr, J Struct Biol. 2008 Feb;161(2):133-43. Epub 2007 Oct 15. PMID:[http:// | Identification of a new quaternary association for legume lectins., Moreno FB, de Oliveira TM, Martil DE, Vicoti MM, Bezerra GA, Abrego JR, Cavada BS, Filgueira de Azevedo W Jr, J Struct Biol. 2008 Feb;161(2):133-43. Epub 2007 Oct 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18068379 18068379] | ||
[[Category: Lotus tetragonolobus]] | [[Category: Lotus tetragonolobus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:40:34 2008'' | ||
Revision as of 14:40, 20 March 2008
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| 2eig, resolution 2.00Å | |||||||||||||
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| Sites: | AC1, AC2, AC3, AC4, AC5, AC6, AC7, AC8, AC9 and BC1 | ||||||||||||
| Ligands: | NAG, MN and CA | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Lotus tetragonolobus seed lectin (Isoform)
Overview
Lotus tetragonolobus lectin (LTA) is a fucose-specific legume lectin. Although several studies report a diverse combination of biological activities for LTA, little is known about the mechanisms involved in l-fucosyl oligosaccharide recognition. The crystal structure of LTA at 2.0A resolution reveals a different legume lectin tetramer. Its structure consists of a homotetramer composed of two back-to-back GS4-like dimers arranged in a new mode, resulting in a novel tetramer. The LTA N-linked carbohydrate at Asn4 and the unusual LTA dimer-dimer interaction are related to its particular mode of tetramerization. In addition, we used small angle X-ray scattering to investigate the quaternary structure of LTA in solution and to compare it to the crystalline structure. Although the crystal structure of LTA has revealed a conserved metal-binding site, its l-fucose-binding site presents some punctual differences. Our investigation of the new tetramer of LTA and its fucose-binding site is essential for further studies related to cross-linking between LTA and complex divalent l-fucosyl carbohydrates.
About this Structure
2EIG is a Single protein structure of sequence from Lotus tetragonolobus. Full crystallographic information is available from OCA.
Reference
Identification of a new quaternary association for legume lectins., Moreno FB, de Oliveira TM, Martil DE, Vicoti MM, Bezerra GA, Abrego JR, Cavada BS, Filgueira de Azevedo W Jr, J Struct Biol. 2008 Feb;161(2):133-43. Epub 2007 Oct 15. PMID:18068379
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