2y0f

STRUCTURE OF GCPE (ISPG) FROM THERMUS THERMOPHILUS HB27

spinqualitylabelsall models 2y0f, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2y0f is a 4 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SF4 Activity: (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase, with EC number 1.17.7.1 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Isoprenoids are biosynthesized via the mevalonate or the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathways the latter being used by most pathogenic bacteria, some parasitic protozoa, plant plastids, but not by animals. We determined the X-ray structure of the homodimeric [4Fe-4S] cluster carrying E-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate synthase (GcpE) of Thermus thermophilus which catalyzes the penultimate reaction of the MEP pathway and is therefore an attractive target for drug development. The [4Fe-4S] cluster ligated to three cysteines and one glutamate is encapsulated at the intersubunit interface. The substrate binding site lies in front of an (alphabeta)(8) barrel. The great [4Fe-4S] cluster-substrate distance implicates large-scale domain rearrangements during the reaction cycle. STRUCTURED SUMMARY: gcpEbinds to gcpE by x-ray crystallography (View interaction). Structure of the E-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate synthase (GcpE) from Thermus thermophilus.,Rekittke I, Nonaka T, Wiesner J, Demmer U, Warkentin E, Jomaa H, Ermler U FEBS Lett. 2010 Dec 15. PMID:21167158 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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