2crx: Difference between revisions

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==Overview==

We have determined the X-ray crystal structures of two DNA Holliday, junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched, structure that occurs as an intermediate in genetic recombination, pathways, including site-specific recombination by the lambda-integrase, family. Cre recombinase is an integrase family member that recombines 34, bp loxP sites in the absence of accessory proteins or auxiliary DNA, sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ, and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ, reveal a nearly planar, twofold-symmetric DNA intermediate that shares, features with both the stacked-X and the square conformations of the HJ, that exist in the unbound state. The structures support a protein-mediated, ..~~. [[http://ispc.weizmann.ac.il/pmbin/getpm?9670032 (full description)]]~~

We have determined the X-ray crystal structures of two DNA Holliday, junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched, structure that occurs as an intermediate in genetic recombination, pathways, including site-specific recombination by the lambda-integrase, family. Cre recombinase is an integrase family member that recombines 34, bp loxP sites in the absence of accessory proteins or auxiliary DNA, sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ, and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ, reveal a nearly planar, twofold-symmetric DNA intermediate that shares, features with both the stacked-X and the square conformations of the HJ, that exist in the unbound state. The structures support a protein-mediated, crossover isomerization of the junction that acts as the switch, responsible for activation and deactivation of recombinase active sites., In this model, a subtle isomerization of the Cre recombinase-HJ quaternary, structure dictates which strands are cleaved during resolution of the, junction via a mechanism that involves neither branch migration nor, helical restacking.

==About this Structure==

2CRX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteriophage_p1 Bacteriophage p1]]. Structure known Active Sites: CIA and CIB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CRX OCA]].

2CRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_p1 Bacteriophage p1]. Structure known Active Sites: CIA and CIB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CRX OCA].

==Reference==

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[[Category: recombination]]

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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:40:05 2007''

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@@ Line 5: / Line 5: @@
 ==Overview==
-We have determined the X-ray crystal structures of two DNA Holliday, junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched, structure that occurs as an intermediate in genetic recombination, pathways, including site-specific recombination by the lambda-integrase, family. Cre recombinase is an integrase family member that recombines 34, bp loxP sites in the absence of accessory proteins or auxiliary DNA, sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ, and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ, reveal a nearly planar, twofold-symmetric DNA intermediate that shares, features with both the stacked-X and the square conformations of the HJ, that exist in the unbound state. The structures support a protein-mediated, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9670032 (full description)]]
+We have determined the X-ray crystal structures of two DNA Holliday, junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched, structure that occurs as an intermediate in genetic recombination, pathways, including site-specific recombination by the lambda-integrase, family. Cre recombinase is an integrase family member that recombines 34, bp loxP sites in the absence of accessory proteins or auxiliary DNA, sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ, and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ, reveal a nearly planar, twofold-symmetric DNA intermediate that shares, features with both the stacked-X and the square conformations of the HJ, that exist in the unbound state. The structures support a protein-mediated, crossover isomerization of the junction that acts as the switch, responsible for activation and deactivation of recombinase active sites., In this model, a subtle isomerization of the Cre recombinase-HJ quaternary, structure dictates which strands are cleaved during resolution of the, junction via a mechanism that involves neither branch migration nor, helical restacking.
 ==About this Structure==
-CRX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacteriophage_p1 Bacteriophage p1]]. Structure known Active Sites: CIA and CIB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CRX OCA]].
+CRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_p1 Bacteriophage p1]. Structure known Active Sites: CIA and CIB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CRX OCA].
 ==Reference==
@@ Line 22: / Line 22: @@
 [[Category: recombination]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:14:02 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 13:40:05 2007''