2ez6: Difference between revisions
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[[Image:2ez6.gif|left|200px]] | [[Image:2ez6.gif|left|200px]] | ||
'''Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing''' | {{Structure | ||
|PDB= 2ez6 |SIZE=350|CAPTION= <scene name='initialview01'>2ez6</scene>, resolution 2.05Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | |||
|GENE= rnc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |||
}} | |||
'''Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2EZ6 is a [ | 2EZ6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZ6 OCA]. | ||
==Reference== | ==Reference== | ||
Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Cell. 2006 Jan 27;124(2):355-66. PMID:[http:// | Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Cell. 2006 Jan 27;124(2):355-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16439209 16439209] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Ribonuclease III]] | [[Category: Ribonuclease III]] | ||
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[[Category: rna processing]] | [[Category: rna processing]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:46:05 2008'' | ||
Revision as of 14:46, 20 March 2008
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| 2ez6, resolution 2.05Å | |||||||||||||
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| Ligands: | MG | ||||||||||||
| Gene: | rnc (Aquifex aeolicus) | ||||||||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of Aquifex aeolicus RNase III (D44N) complexed with product of double-stranded RNA processing
Overview
Members of the ribonuclease III (RNase III) family are double-stranded RNA (dsRNA) specific endoribonucleases characterized by a signature motif in their active centers and a two-base 3' overhang in their products. While Dicer, which produces small interfering RNAs, is currently the focus of intense interest, the structurally simpler bacterial RNase III serves as a paradigm for the entire family. Here, we present the crystal structure of an RNase III-product complex, the first catalytic complex observed for the family. A 7 residue linker within the protein facilitates induced fit in protein-RNA recognition. A pattern of protein-RNA interactions, defined by four RNA binding motifs in RNase III and three protein-interacting boxes in dsRNA, is responsible for substrate specificity, while conserved amino acid residues and divalent cations are responsible for scissile-bond cleavage. The structure reveals a wealth of information about the mechanism of RNA hydrolysis that can be extrapolated to other RNase III family members.
About this Structure
2EZ6 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Cell. 2006 Jan 27;124(2):355-66. PMID:16439209
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