Structural highlights
Publication Abstract from PubMed
A novel bifunctional bicyclic inhibitor has been created that combines features both from the Bowman-Birk inhibitor (BBI) proteins, which have two distinct inhibitory sites, and from sunflower trypsin inhibitor-1 (SFTI-1), which has a compact bicyclic structure. The inhibitor was designed by fusing together a pair of reactive loops based on a sequence derived from SFTI-1 to create a backbone-cyclized disulfide-bridged 16-mer peptide. This peptide has two symmetrically spaced trypsin binding sites. Its synthesis and biological activity have been reported in a previous communication [Jaulent and Leatherbarrow, 2004, PEDS 17, 681]. In the present study we have examined the three-dimensional structure of the molecule. We find that the new inhibitor, which has a symmetrical 8-mer half-cystine CTKSIPP'I' motif repeated through a C2 symmetry axis also shows a complete symmetry in its three-dimensional structure. Each of the two loops adopts the expected canonical conformation common to all BBIs as well as SFTI-1. We also find that the inhibitor displays a strong and unique structural identity, with a notable lack of minor conformational isomers that characterise most reactive site loop mimics examined to date as well as SFTI-1. This suggests that the presence of the additional cyclic loop acts to restrict conformational mobility and that the deliberate introduction of cyclic symmetry may offer a general route to locking the conformation of beta-hairpin structures.
Solution structure of a novel C2-symmetrical bifunctional bicyclic inhibitor based on SFTI-1.,Jaulent AM, Brauer AB, Matthews SJ, Leatherbarrow RJ J Biomol NMR. 2005 Sep;33(1):57-62. PMID:16222558[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jaulent AM, Brauer AB, Matthews SJ, Leatherbarrow RJ. Solution structure of a novel C2-symmetrical bifunctional bicyclic inhibitor based on SFTI-1. J Biomol NMR. 2005 Sep;33(1):57-62. PMID:16222558 doi:10.1007/s10858-005-1210-9
