2bmo: Difference between revisions

Nitroaromatic compounds are used extensively in many industrial processes, and have been released into the environment where they are considered, environmental pollutants. Nitroaromatic compounds, in general, are, resistant to oxidative attack due to the electron-withdrawing nature of, the nitro groups and the stability of the benzene ring. However, the, bacterium Comamonas sp. strain JS765 can grow with nitrobenzene as a sole, source of carbon, nitrogen and energy. Biodegradation is initiated by the, nitrobenzene dioxygenase (NBDO) system. We have determined the structure, of NBDO, which has a hetero-hexameric structure similar to that of several, other Rieske non-heme iron dioxygenases. The catalytic subunit contains a, Rieske iron-sulfur center and an active-site mononuclear iron atom. The, structures of complexes with substrates nitrobenzene and 3-nitrotoluene, reveal the structural basis for its activity with nitroarenes. The, substrate pocket contains an asparagine residue that forms a hydrogen bond, to the nitro-group of the substrate, and orients the substrate in relation, to the active-site mononuclear iron atom, positioning the molecule for, oxidation at the nitro-substituted carbon.

2BMO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Comamonas_sp. Comamonas sp.] with FE, NI, FES, EDO and EOH as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BMO OCA].  

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