2fqn: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2fqn.gif|left|200px]] | [[Image:2fqn.gif|left|200px]] | ||
'''Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site''' | {{Structure | ||
|PDB= 2fqn |SIZE=350|CAPTION= <scene name='initialview01'>2fqn</scene>, resolution 2.300Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2FQN is a [ | 2FQN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQN OCA]. | ||
==Reference== | ==Reference== | ||
Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site., Kondo J, Urzhumtsev A, Westhof E, Nucleic Acids Res. 2006 Feb 1;34(2):676-85. Print 2006. PMID:[http:// | Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site., Kondo J, Urzhumtsev A, Westhof E, Nucleic Acids Res. 2006 Feb 1;34(2):676-85. Print 2006. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16452297 16452297] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Kondo, J.]] | [[Category: Kondo, J.]] | ||
| Line 23: | Line 32: | ||
[[Category: rrna]] | [[Category: rrna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:55:40 2008'' | ||
Revision as of 14:55, 20 March 2008
| |||||||||||||
| 2fqn, resolution 2.300Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | MG and NCO | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site
Overview
The decoding A site of the small ribosomal subunit is an RNA molecular switch, which monitors codon-anticodon interactions to guarantee translation fidelity. We have solved the crystal structure of an RNA fragment containing two Homo sapiens cytoplasmic A sites. Each of the two A sites presents a different conformational state. In one state, adenines A1492 and A1493 are fully bulged-out with C1409 forming a wobble-like pair to A1491. In the second state, adenines A1492 and A1493 form non-Watson-Crick pairs with C1409 and G1408, respectively while A1491 bulges out. The first state of the eukaryotic A site is, thus, basically the same as in the bacterial A site with bulging A1492 and A1493. It is the state used for recognition of the codon/anticodon complex. On the contrary, the second state of the H.sapiens cytoplasmic A site is drastically different from any of those observed for the bacterial A site without bulging A1492 and A1493.
About this Structure
2FQN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site., Kondo J, Urzhumtsev A, Westhof E, Nucleic Acids Res. 2006 Feb 1;34(2):676-85. Print 2006. PMID:16452297
Page seeded by OCA on Thu Mar 20 16:55:40 2008
