Resolvase: Difference between revisions
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{{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE=Resolvase/Cv/1 |right|CAPTION=Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]] }} | {{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE=Resolvase/Cv/1 |right|CAPTION=Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]] }} | ||
[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. The '''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. '''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). '''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. '''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:11726496</ref>. | [[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. The '''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. '''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). '''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. '''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:11726496</ref>. '''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions. | ||
==3D structures of resolvase== | ==3D structures of resolvase== | ||
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**[[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br /> | **[[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br /> | ||
*Hin recombinase | |||
**[[1hcr]], [[1ijw]], [[1ijj6]], [[1jj8]], [[1jko]], [[1jkp]], [[1jkq]], [[1jkr]] – HRec + DNA – synthetic <br /> | |||
* Integrase | * Integrase | ||
