Practical Guide to Homology Modeling: Difference between revisions
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===Evolutionary Conservation=== | |||
Patches of highly conserved amino acids in a homology model can be very informative, as such patches indicate functional sites. | |||
*Go to the ConSurf Server: [http://consurf.tau.ac.il ConSurf.tau.ac.il]. | |||
*Click ''Amino Acids''. | |||
*Click YES there is a known protein structure. | |||
*Click ''Choose File'' to upload your homology model. Click ''Next''. | |||
*Select chain A. | |||
*Select NO you have not prepared a Multiple Sequence Alignment (MSA) that you wish to upload. | |||
*Change ''number of iterations'' to 1. | |||
*Check 'Let me select the sequences'. Leave all other settings at their defaults. | |||
*Enter a job title and your email address, then click the ''Submit'' button. The first step, gathering similar sequences, typically takes less than 5 minutes. | |||
*When the sequences are gathered, you will see <font color="green">SELECT SEQUENCES</font>. | |||
==See Also== | ==See Also== | ||
