2fx0: Difference between revisions
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'''Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator''' | {{Structure | ||
|PDB= 2fx0 |SIZE=350|CAPTION= <scene name='initialview01'>2fx0</scene>, resolution 2.40Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= hlyIIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus]) | |||
}} | |||
'''Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2FX0 is a [ | 2FX0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FX0 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of Bacillus cereus HlyIIR, a transcriptional regulator of the gene for pore-forming toxin hemolysin II., Kovalevskiy OV, Lebedev AA, Surin AK, Solonin AS, Antson AA, J Mol Biol. 2007 Jan 19;365(3):825-34. Epub 2006 Oct 26. PMID:[http:// | Crystal structure of Bacillus cereus HlyIIR, a transcriptional regulator of the gene for pore-forming toxin hemolysin II., Kovalevskiy OV, Lebedev AA, Surin AK, Solonin AS, Antson AA, J Mol Biol. 2007 Jan 19;365(3):825-34. Epub 2006 Oct 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17097673 17097673] | ||
[[Category: Bacillus cereus]] | [[Category: Bacillus cereus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcriptional regulator]] | [[Category: transcriptional regulator]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:57:48 2008'' | ||
Revision as of 14:57, 20 March 2008
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| 2fx0, resolution 2.40Å | |||||||||||||
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| Gene: | hlyIIR (Bacillus cereus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of HlyIIR, a Hemolysin II transcriptional Regulator
Overview
Production of Bacillus cereus and Bacillus anthracis toxins is controlled by a number of transcriptional regulators. Here we report the crystal structure of B. cereus HlyIIR, a regulator of the gene encoding the pore-forming toxin hemolysin II. We show that HlyIIR forms a tight dimer with a fold and overall architecture similar to the TetR family of repressors. A remarkable feature of the structure is a large internal cavity with a volume of 550 A(3) suggesting that the activity of HlyIIR is modulated by binding of a ligand, which triggers the toxin production. Virtual ligand library screening shows that this pocket can accommodate compounds with molecular masses of up to 400-500 Da. Based on structural data and previous biochemical evidence, we propose a model for HlyIIR interaction with the DNA.
About this Structure
2FX0 is a Single protein structure of sequence from Bacillus cereus. Full crystallographic information is available from OCA.
Reference
Crystal structure of Bacillus cereus HlyIIR, a transcriptional regulator of the gene for pore-forming toxin hemolysin II., Kovalevskiy OV, Lebedev AA, Surin AK, Solonin AS, Antson AA, J Mol Biol. 2007 Jan 19;365(3):825-34. Epub 2006 Oct 26. PMID:17097673
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