Sandbox Reserved 975: Difference between revisions
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There are two cis-prolines Pro69 and Pro79 that play an important role in the structure of the protein. | There are two cis-prolines Pro69 and Pro79 that play an important role in the structure of the protein. | ||
== Function == | == Function == | ||
The active site residue Cys 93 is located in a crevice formed by a loop between β4 and α2 (85-102) and another one between α2 and α3 (residues 122-130) | |||
5 residues are the most likely to participate to the catalytic action because they are located within 6 Å of the sulfhydryl group of the accepting cysteine and that their side chains are oriented toward the Cysteine : Asn85, Tyr87, Glu98, Lys101, and Asp12 | |||
15 residues are well conserved among the UBC family : Gly47, Lys48, Gly56, Tyr68, Pro69, Pro73, Phe77, His83, Pro84, Asn85, Gly90, Trp103, Pro105, Leu120, and Pro12 | |||
Most of them are non polar and they are unlikely to have a direct role in the catalytic action but they are probably positionned to maintain the special configuration of the active site. | |||
== Interaction with the substrates == | == Interaction with the substrates == | ||
