Sandbox Reserved 962: Difference between revisions
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<scene name='60/604481/Interaction_sah/1'>10 amino acids</scene> (Lys54, Gly72, Asp78, Asp94, Ile95, Asp122, Ser124, Gln140, Phe141, Ser142) are involved in the stabilisation of AdoHcys. | <scene name='60/604481/Interaction_sah/1'>10 amino acids</scene> (Lys54, Gly72, Asp78, Asp94, Ile95, Asp122, Ser124, Gln140, Phe141, Ser142) are involved in the stabilisation of AdoHcys. | ||
The interactions between AdoHcys and the enzyme are made of : hydrogen bonds, Van der Walls interactions,electrostatic interaction and water mediated contact. | The interactions between AdoHcys and the enzyme are made of : hydrogen bonds, Van der Walls interactions,electrostatic interaction and water mediated contact. | ||
<ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | <ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | ||
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Indeed the enzyme does not stabilize the transition state of the chemical reaction, does not promote the activation of the nucleophile or the explusion of the leaving group. mRNA Cap Methyltransferase brings the two substrates closer and orientates the substrates to facilitate the methyl transfer. | Indeed the enzyme does not stabilize the transition state of the chemical reaction, does not promote the activation of the nucleophile or the explusion of the leaving group. mRNA Cap Methyltransferase brings the two substrates closer and orientates the substrates to facilitate the methyl transfer. | ||
== References == | == References == | ||
<references/> | <references/> | ||
