Sandbox Reserved 962: Difference between revisions
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<scene name='60/604481/Gtg/3'>A cap analog</scene> binds to the enzyme in a pocket near the AdoHcy. | <scene name='60/604481/Gtg/3'>A cap analog</scene> binds to the enzyme in a pocket near the AdoHcy. | ||
<scene name='60/604481/Interaction_gtg/1'>6 amino acids</scene> (Tyr 145, Leu216, Leu217, Asp218, Ser219, Tyr284) are involded in the binding of the cap analog. | <scene name='60/604481/Interaction_gtg/1'>6 amino acids</scene> (Tyr 145, Leu216, Leu217, Asp218, Ser219, Tyr284) are involded in the binding of the cap analog. | ||
The cap makes Van der Walls contacts, hydrogen bond and water mediated bond. | The cap makes Van der Walls contacts thanks to side chain from Leu216, Leu217, Asp218, Ser219, Tyr284 makes a hydrogen bond and Tyr145 a water mediated bond. | ||
<ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | <ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | ||
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AdoHcys is in a pocket formed by amino acids of segment 2. | AdoHcys is in a pocket formed by amino acids of segment 2. | ||
<scene name='60/604481/Interaction_sah/1'>10 amino acids</scene> (Lys54, Gly72, Asp78, Asp94, Ile95, Asp122, Ser124, Gln140, Phe141, Ser142) are involved in the stabilisation of AdoHcys. | <scene name='60/604481/Interaction_sah/1'>10 amino acids</scene> (Lys54, Gly72, Asp78, Asp94, Ile95, Asp122, Ser124, Gln140, Phe141, Ser142) are involved in the stabilisation of AdoHcys. | ||
The interactions between AdoHcys and the enzyme are made of : hydrogen bonds, Van der Walls interactions,electrostatic interaction | The interactions between AdoHcys and the enzyme are made of : {{clear}} | ||
hydrogen bonds are made by Asp122, Asp 94, Gln140, Gly72, Lys54 {{clear}} | |||
Van der Walls interactions are made by Ser142, Tyr124 and Ile95 {{clear}} | |||
an electrostatic interaction is made by Gln140, Phe141{{clear}} | |||
a water mediated contact is made by Asp78. | |||
<ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | <ref name="Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD. Structure and mechanism of mRNA cap (guanine-N7) methyltransferase. Mol Cell. 2004 Jan 16;13(1):77-89. ">PMID:14731396</ref> | ||
