2yn2: Difference between revisions
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==Huf protein - paralogue of the tau55 histidine phosphatase domain== | |||
<StructureSection load='2yn2' size='340' side='right' caption='[[2yn2]], [[Resolution|resolution]] 2.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2yn2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YN2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YN2 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yn2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yn2 RCSB], [http://www.ebi.ac.uk/pdbsum/2yn2 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Saccharomyces cerevisiae tau55, a subunit of the RNA polymerase III-specific general transcription factor TFIIIC, comprises an N-terminal histidine phosphatase domain (tau55-HPD) whose catalytic activity and cellular function is poorly understood. We solved the crystal structures of tau55-HPD and its closely related paralogue Huf and used in silico docking methods to identify phospho-serine and phospho-tyrosine containing peptides as possible substrates that were subsequently validated using in vitro phosphatase assays. A comparative phospho-proteomic study identified additional phosphopeptides as possible targets, which show the involvement of these two phosphatases in the regulation of a variety of cellular functions. Our results identify tau55-HPD and Huf as bona fide protein phosphatases, characterize their substrate specificities and provide a small set of regulated phosphosite targets in vivo. | |||
Structural and functional characterization of a phosphatase domain within yeast general transcription factor TFIIIC.,Taylor NM, Glatt S, Hennrich ML, von Scheven G, Grotsch H, Fernandez-Tornero C, Rybin V, Gavin AC, Kolb P, Muller CW J Biol Chem. 2013 Apr 8. PMID:23569204<ref>PMID:23569204</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Saccharomyces cerevisiae s288c]] | [[Category: Saccharomyces cerevisiae s288c]] | ||
[[Category: Fernandez-Tornero, C | [[Category: Fernandez-Tornero, C]] | ||
[[Category: Gavin, A C | [[Category: Gavin, A C]] | ||
[[Category: Glatt, S | [[Category: Glatt, S]] | ||
[[Category: Grotsch, H | [[Category: Grotsch, H]] | ||
[[Category: Hennrich, M | [[Category: Hennrich, M]] | ||
[[Category: Kolb, P | [[Category: Kolb, P]] | ||
[[Category: Muller, C W | [[Category: Muller, C W]] | ||
[[Category: Rybin, V | [[Category: Rybin, V]] | ||
[[Category: Scheven, G von | [[Category: Scheven, G von]] | ||
[[Category: Taylor, N M.I | [[Category: Taylor, N M.I]] | ||
[[Category: Histidine phosphatase domain]] | [[Category: Histidine phosphatase domain]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Phosphoglycerate mutase domain]] | [[Category: Phosphoglycerate mutase domain]] | ||
Revision as of 21:40, 3 January 2015
Huf protein - paralogue of the tau55 histidine phosphatase domain
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