3r4y: Difference between revisions
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[[ | ==Crystal structure of alpha-neoagarobiose hydrolase (ALPHA-NABH) from Saccharophagus degradans 2-40== | ||
<StructureSection load='3r4y' size='340' side='right' caption='[[3r4y]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3r4y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharophagus_degradans Saccharophagus degradans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R4Y FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r4z|3r4z]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sde_2657 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=86304 Saccharophagus degradans])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r4y RCSB], [http://www.ebi.ac.uk/pdbsum/3r4y PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In agarolytic microorganisms, alpha-neoagarobiose hydrolase (NABH) is an essential enzyme to metabolize agar because it converts alpha-neoagarobiose (O-3,6-anhydro-alpha-l-galactopyranosyl-(1,3)-d-galactose) into fermentable monosaccharides (d-galactose and 3,6-anhydro-l-galactose) in the agarolytic pathway. NABH can be divided into two biological classes by its cellular location. Here, we describe a structure and function of cytosolic NABH from Saccharophagus degradans 2-40 in a native protein and d-galactose complex determined at 2.0 and 1.55 A, respectively. The overall fold is organized in an N-terminal helical extension and a C-terminal five-bladed beta-propeller catalytic domain. The structure of the enzyme-ligand (d-galactose) complex predicts a +1 subsite in the substrate binding pocket. The structural features may provide insights for the evolution and classification of NABH in agarolytic pathways. | |||
Crystal structure of a key enzyme in the agarolytic pathway, alpha-neoagarobiose hydrolase from Saccharophagus degradans 2-40.,Ha SC, Lee S, Lee J, Kim HT, Ko HJ, Kim KH, Choi IG Biochem Biophys Res Commun. 2011 Aug 26;412(2):238-44. Epub 2011 Jul 23. PMID:21810409<ref>PMID:21810409</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Saccharophagus degradans]] | [[Category: Saccharophagus degradans]] | ||
[[Category: Bang, W G | [[Category: Bang, W G]] | ||
[[Category: Choi, I G | [[Category: Choi, I G]] | ||
[[Category: Ha, S C | [[Category: Ha, S C]] | ||
[[Category: Kim, K H | [[Category: Kim, K H]] | ||
[[Category: Kim, S H | [[Category: Kim, S H]] | ||
[[Category: Lee, J Y | [[Category: Lee, J Y]] | ||
[[Category: Lee, S | [[Category: Lee, S]] | ||
[[Category: Shin, D H | [[Category: Shin, D H]] | ||
[[Category: 6-anhydro-l-galactose]] | [[Category: 6-anhydro-l-galactose]] | ||
[[Category: Agar metabolism]] | [[Category: Agar metabolism]] | ||
