2ghv: Difference between revisions
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[[Image:2ghv.gif|left|200px]] | [[Image:2ghv.gif|left|200px]] | ||
'''Crystal structure of SARS spike protein receptor binding domain''' | {{Structure | ||
|PDB= 2ghv |SIZE=350|CAPTION= <scene name='initialview01'>2ghv</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Human SARS coronavirus]) | |||
}} | |||
'''Crystal structure of SARS spike protein receptor binding domain''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GHV is a [ | 2GHV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:[http:// | Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16954221 16954221] | ||
[[Category: Human sars coronavirus]] | [[Category: Human sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Sui, J.]] | [[Category: Sui, J.]] | ||
[[Category: s protein]] | [[Category: s protein]] | ||
[[Category: | [[Category: sar]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:04:53 2008'' | ||
Revision as of 15:04, 20 March 2008
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| 2ghv, resolution 2.20Å | |||||||||||||
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| Gene: | S (Human SARS coronavirus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of SARS spike protein receptor binding domain
Overview
Severe acute respiratory syndrome (SARS) is a newly emerged infectious disease that caused pandemic spread in 2003. The etiological agent of SARS is a novel coronavirus (SARS-CoV). The coronaviral surface spike protein S is a type I transmembrane glycoprotein that mediates initial host binding via the cell surface receptor angiotensin-converting enzyme 2 (ACE2), as well as the subsequent membrane fusion events required for cell entry. Here we report the crystal structure of the S1 receptor binding domain (RBD) in complex with a neutralizing antibody, 80R, at 2.3 A resolution, as well as the structure of the uncomplexed S1 RBD at 2.2 A resolution. We show that the 80R-binding epitope on the S1 RBD overlaps very closely with the ACE2-binding site, providing a rationale for the strong binding and broad neutralizing ability of the antibody. We provide a structural basis for the differential effects of certain mutations in the spike protein on 80R versus ACE2 binding, including escape mutants, which should facilitate the design of immunotherapeutics to treat a future SARS outbreak. We further show that the RBD of S1 forms dimers via an extensive interface that is disrupted in receptor- and antibody-bound crystal structures, and we propose a role for the dimer in virus stability and infectivity.
About this Structure
2GHV is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.
Reference
Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:16954221
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