2gsk: Difference between revisions
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[[Image:2gsk.gif|left|200px]] | [[Image:2gsk.gif|left|200px]] | ||
'''Structure of the BtuB:TonB Complex''' | {{Structure | ||
|PDB= 2gsk |SIZE=350|CAPTION= <scene name='initialview01'>2gsk</scene>, resolution 2.10Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CNC:CO-CYANOCOBALAMIN'>CNC</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene> and <scene name='pdbligand=HEX:HEXANE'>HEX</scene> | |||
|ACTIVITY= | |||
|GENE= btuB, bfe, cer, dcrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), tonB, exbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Structure of the BtuB:TonB Complex''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GSK is a [ | 2GSK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GSK OCA]. | ||
==Reference== | ==Reference== | ||
Outer membrane active transport: structure of the BtuB:TonB complex., Shultis DD, Purdy MD, Banchs CN, Wiener MC, Science. 2006 Jun 2;312(5778):1396-9. PMID:[http:// | Outer membrane active transport: structure of the BtuB:TonB complex., Shultis DD, Purdy MD, Banchs CN, Wiener MC, Science. 2006 Jun 2;312(5778):1396-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16741124 16741124] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: tonb]] | [[Category: tonb]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:08:31 2008'' | ||
Revision as of 15:08, 20 March 2008
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| 2gsk, resolution 2.10Å | |||||||||||||
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| Ligands: | CA, CNC, LDA, OCT and HEX | ||||||||||||
| Gene: | btuB, bfe, cer, dcrC (Escherichia coli), tonB, exbA (Escherichia coli) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of the BtuB:TonB Complex
Overview
In Gram-negative bacteria, the import of essential micronutrients across the outer membrane requires a transporter, an electrochemical gradient of protons across the inner membrane, and an inner membrane protein complex (ExbB, ExbD, TonB) that couples the proton-motive force to the outer membrane transporter. The inner membrane protein TonB binds directly to a conserved region, called the Ton-box, of the transporter. We solved the structure of the cobalamin transporter BtuB in complex with the C-terminal domain of TonB. In contrast to its conformations in the absence of TonB, the Ton-box forms a beta strand that is recruited to the existing beta sheet of TonB, which is consistent with a mechanical pulling model of transport.
About this Structure
2GSK is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Outer membrane active transport: structure of the BtuB:TonB complex., Shultis DD, Purdy MD, Banchs CN, Wiener MC, Science. 2006 Jun 2;312(5778):1396-9. PMID:16741124
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