4bhl: Difference between revisions
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==Crystal structure of Litopenaeus vannamei arginine kinase in binary complex with arginine== | |||
=== | <StructureSection load='4bhl' size='340' side='right' caption='[[4bhl]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4bhl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Litopenaeus_vannamei Litopenaeus vannamei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BHL FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arginine_kinase Arginine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.3 2.7.3.3] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bhl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bhl RCSB], [http://www.ebi.ac.uk/pdbsum/4bhl PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Arginine kinase (AK) is a key enzyme for energetic balance in invertebrates. Although AK is a well-studied system that provides fast energy to invertebrates using the phosphagen phospho-arginine, the structural details on the AK-arginine binary complex interaction remain unclear. Herein, we determined two crystal structures of the Pacific whiteleg shrimp (Litopenaeus vannamei) arginine kinase, one in binary complex with arginine (LvAK-Arg) and a ternary transition state analog complex (TSAC). We found that the arginine guanidinium group makes ionic contacts with Glu225, Cys271 and a network of ordered water molecules. On the zwitterionic side of the amino acid, the backbone amide nitrogens of Gly64 and Val65 coordinate the arginine carboxylate. Glu314, one of proposed acid-base catalytic residues, did not interact with arginine in the binary complex. This residue is located in the flexible loop 310-320 that covers the active site and only stabilizes in the LvAK-TSAC. This is the first binary complex crystal structure of a guanidine kinase in complex with the guanidine substrate and could give insights into the nature of the early steps of phosphagen biosynthesis. | |||
Crystal structure of shrimp arginine kinase in binary complex with arginine-a molecular view of the phosphagen precursor binding to the enzyme.,Lopez-Zavala AA, Garcia-Orozco KD, Carrasco-Miranda JS, Sugich-Miranda R, Velazquez-Contreras EF, Criscitiello MF, Brieba LG, Rudino-Pinera E, Sotelo-Mundo RR J Bioenerg Biomembr. 2013 Jul 20. PMID:23873077<ref>PMID:23873077</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Arginine kinase]] | [[Category: Arginine kinase]] | ||
[[Category: Litopenaeus vannamei]] | [[Category: Litopenaeus vannamei]] | ||
[[Category: Brieba, L G | [[Category: Brieba, L G]] | ||
[[Category: Carrasco-Miranda, J S | [[Category: Carrasco-Miranda, J S]] | ||
[[Category: Criscitiello, M F | [[Category: Criscitiello, M F]] | ||
[[Category: Garcia-Orozco, K D | [[Category: Garcia-Orozco, K D]] | ||
[[Category: Hernandez-Flores, J M | [[Category: Hernandez-Flores, J M]] | ||
[[Category: Lopez-Zavala, A A | [[Category: Lopez-Zavala, A A]] | ||
[[Category: Rudino-Pinera, E | [[Category: Rudino-Pinera, E]] | ||
[[Category: Sotelo-Mundo, R R | [[Category: Sotelo-Mundo, R R]] | ||
[[Category: Sugich-Miranda, R | [[Category: Sugich-Miranda, R]] | ||
[[Category: Velazquez-Contreras, E F | [[Category: Velazquez-Contreras, E F]] | ||
[[Category: Binary complex]] | [[Category: Binary complex]] | ||
[[Category: Phosphagen]] | [[Category: Phosphagen]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
