4di3: Difference between revisions

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[[Image:4di3.png|left|200px]]
==Crystal structure of a 2:1 complex of Treponema pallidum TatP(T) (Tp0957) bound to TatT (Tp0956)==
<StructureSection load='4di3' size='340' side='right' caption='[[4di3]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4di3]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum_subsp._pallidum Treponema pallidum subsp. pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DI3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DI3 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3u64|3u64]], [[3u65|3u65]], [[3di4|3di4]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TP_0957 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161 Treponema pallidum subsp. pallidum]), TP_0956 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161 Treponema pallidum subsp. pallidum])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4di3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4di3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4di3 RCSB], [http://www.ebi.ac.uk/pdbsum/4di3 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tripartite ATP-independent periplasmic transporters (TRAP-Ts) are bacterial transport systems that have been implicated in the import of small molecules into the cytoplasm. A newly discovered subfamily of TRAP-Ts [tetratricopeptide repeat-protein associated TRAP transporters (TPATs)] has four components. Three are common to both TRAP-Ts and TPATs: the P component, a ligand-binding protein, and a transmembrane symporter apparatus comprising the M and Q components (M and Q are sometimes fused to form a single polypeptide). TPATs are distinguished from TRAP-Ts by the presence of a unique protein called the "T component". In Treponema pallidum, this protein (TatT) is a water-soluble trimer whose protomers are each perforated by a pore. Its respective P component (TatP(T)) interacts with the TatT in vitro and in vivo. In this work, we further characterized this interaction. Co-crystal structures of two complexes between the two proteins confirm that up to three monomers of TatP(T) can bind to the TatT trimer. A putative ligand-binding cleft of TatP(T) aligns with the pore of TatT, strongly suggesting ligand transfer between T and P(T). We used a combination of site-directed mutagenesis and analytical ultracentrifugation to derive thermodynamic parameters for the interactions. These observations confirm that the observed crystallographic interface is recapitulated in solution. These results prompt a hypothesis of the molecular mechanism(s) of hydrophobic ligand transport by the TPATs.


{{STRUCTURE_4di3|  PDB=4di3  |  SCENE=  }}
Structural and Thermodynamic Characterization of the Interaction between Two Periplasmic Treponema pallidum Lipoproteins that are Components of a TPR-Protein-Associated TRAP Transporter (TPAT).,Brautigam CA, Deka RK, Schuck P, Tomchick DR, Norgard MV J Mol Biol. 2012 Apr 11. PMID:22504226<ref>PMID:22504226</ref>


===Crystal structure of a 2:1 complex of Treponema pallidum TatP(T) (Tp0957) bound to TatT (Tp0956)===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_22504226}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[4di3]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum_subsp._pallidum Treponema pallidum subsp. pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DI3 OCA].
</StructureSection>
 
==Reference==
<ref group="xtra">PMID:022504226</ref><references group="xtra"/>
[[Category: Treponema pallidum subsp. pallidum]]
[[Category: Treponema pallidum subsp. pallidum]]
[[Category: Brautigam, C A.]]
[[Category: Brautigam, C A]]
[[Category: Deka, R K.]]
[[Category: Deka, R K]]
[[Category: Norgard, M V.]]
[[Category: Norgard, M V]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
[[Category: Tpat]]
[[Category: Tpat]]
[[Category: Transport protein]]
[[Category: Transport protein]]
[[Category: Trap transporter]]
[[Category: Trap transporter]]
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