4hu8: Difference between revisions
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==Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut== | |||
<StructureSection load='4hu8' size='340' side='right' caption='[[4hu8]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4hu8]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Globitermes_brachycerastes Globitermes brachycerastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HU8 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hu8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hu8 RCSB], [http://www.ebi.ac.uk/pdbsum/4hu8 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial Ig-like (Big) domains are commonly distributed in glycoside hydrolases (GH), but their structure and function remains undefined. Xylanase is a GH, and catalyzes the hydrolysis of the internal beta-xylosidic linkages of xylan. In this study, we report the molecular cloning, biochemical and biophysical characterization, and crystal structure of a termite gut bacterial xylanase, Xyl-ORF19, which was derived from gut bacteria of a wood-feeding termite (Globitermes brachycerastes). The protein architecture of Xyl-ORF19 reveals that it has two domains, a C-terminal GH10 catalytic domain and an N-terminal Big_2 non-catalytic domain. The catalytic domain folds in an (alpha/beta)8 barrel as most GH10 xylanases do, but it has two extra beta-strands. The non-catalytic domain is structurally similar to an immunoglobulin-like domain of intimins. The recombinant enzyme without the non-catalytic domain has fairly low catalytic activity, and is different from the full-length enzyme in kinetic parameters, pH and temperature profiles, which suggests the non-catalytic domain could affect the enzyme biochemical and biophysical properties as well as the role for enzyme localization. This study provides a molecular basis for future efforts in xylanase bioengineering. Biotechnol. Bioeng. 2013;9999: 1-11. (c) 2013 Wiley Periodicals, Inc. | |||
Biochemical characterization and crystal structure of a GH10 xylanase from termite gut bacteria reveal a novel structural feature and significance of its bacterial Ig-like domain.,Han Q, Liu N, Robinson H, Cao L, Qian C, Wang Q, Xie L, Ding H, Wang Q, Huang Y, Li J, Zhou Z Biotechnol Bioeng. 2013 Jun 21. doi: 10.1002/bit.24982. PMID:23794438<ref>PMID:23794438</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Endo-1,4-beta-xylanase]] | [[Category: Endo-1,4-beta-xylanase]] | ||
[[Category: Globitermes brachycerastes]] | [[Category: Globitermes brachycerastes]] | ||
[[Category: Cao, L | [[Category: Cao, L]] | ||
[[Category: Ding, H | [[Category: Ding, H]] | ||
[[Category: Han, Q | [[Category: Han, Q]] | ||
[[Category: Huang, Y | [[Category: Huang, Y]] | ||
[[Category: Li, J | [[Category: Li, J]] | ||
[[Category: Liu, N | [[Category: Liu, N]] | ||
[[Category: Qian, C | [[Category: Qian, C]] | ||
[[Category: Robinson, H | [[Category: Robinson, H]] | ||
[[Category: Wang, Q | [[Category: Wang, Q]] | ||
[[Category: Xie, L | [[Category: Xie, L]] | ||
[[Category: Zhou, Z | [[Category: Zhou, Z]] | ||
[[Category: Bacterial ig-like domain]] | [[Category: Bacterial ig-like domain]] | ||
[[Category: Big2]] | [[Category: Big2]] | ||
[[Category: Glycoside hydrolase]] | [[Category: Glycoside hydrolase]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 12:41, 4 January 2015
Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut
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