2gyd: Difference between revisions
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[[Image:2gyd.gif|left|200px]] | [[Image:2gyd.gif|left|200px]] | ||
'''Complex of equine apoferritin with the H-diaziflurane photolabeling reagent''' | {{Structure | ||
|PDB= 2gyd |SIZE=350|CAPTION= <scene name='initialview01'>2gyd</scene>, resolution 1.720Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> and <scene name='pdbligand=DFE:2-(1,1-DIFLUOROETHOXY)-1,1,1-TRIFLUOROETHANE'>DFE</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Complex of equine apoferritin with the H-diaziflurane photolabeling reagent''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GYD is a [ | 2GYD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GYD OCA]. | ||
==Reference== | ==Reference== | ||
Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions., Xi J, Liu R, Rossi MJ, Yang J, Loll PJ, Dailey WP, Eckenhoff RG, ACS Chem Biol. 2006 Jul 21;1(6):377-84. PMID:[http:// | Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions., Xi J, Liu R, Rossi MJ, Yang J, Loll PJ, Dailey WP, Eckenhoff RG, ACS Chem Biol. 2006 Jul 21;1(6):377-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17163775 17163775] | ||
[[Category: Equus caballus]] | [[Category: Equus caballus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: DFE]] | [[Category: DFE]] | ||
[[Category: anesthetic]] | [[Category: anesthetic]] | ||
[[Category: helical | [[Category: helical bundle]] | ||
[[Category: isoflurane]] | [[Category: isoflurane]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:10:24 2008'' | ||
Revision as of 15:10, 20 March 2008
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| 2gyd, resolution 1.720Å | |||||||||||||
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| Ligands: | CD and DFE | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Complex of equine apoferritin with the H-diaziflurane photolabeling reagent
Overview
The difficulty in obtaining binding target and site information for low-affinity drugs, like the inhaled anesthetics, has limited identification of their molecular effectors. Because such information can be provided by photoactive analogues, we designed, synthesized, and characterized a novel diazirnyl haloether that closely mimics isoflurane, the most widely used clinical general anesthetic. This compound, H-diaziflurane, is a nontoxic, potent anesthetic that potentiates GABA-gated ion channels in primary cultures of hippocampal neurons. Calorimetric and structural characterizations show that H-diaziflurane binds a model anesthetic host protein with similar energetics as isoflurane and forms photoadducts with residues lining the isoflurane binding site. H-diaziflurane will be immediately useful for identifying targets and sites important for the molecular pharmacology of the inhaled haloether anesthetics.
About this Structure
2GYD is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions., Xi J, Liu R, Rossi MJ, Yang J, Loll PJ, Dailey WP, Eckenhoff RG, ACS Chem Biol. 2006 Jul 21;1(6):377-84. PMID:17163775
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