4kva: Difference between revisions
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==GTPase domain of Septin 10 from Schistosoma mansoni in complex with GTP== | |||
<StructureSection load='4kva' size='340' side='right' caption='[[4kva]], [[Resolution|resolution]] 2.14Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4kva]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KVA FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kv9|4kv9]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">septin 10, Smp_029890 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6183 Blood fluke])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kva OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kva RCSB], [http://www.ebi.ac.uk/pdbsum/4kva PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Septins are filament forming GTP-binding proteins involved in important cellular events such as cytokinesis, barrier formation and membrane remodeling. Here, we present two crystal structures of the GTPase domain of a Schistosoma mansoni septin (SmSEPT10); one bound to GDP and the other to GTP. The structures have been solved at an unprecedented resolution for septins (1.93 and 2.1 A respectively) which has allowed for unambiguous structural assignment of regions previously poorly defined. Consequently we provide a reliable model for functional interpretation and a solid foundation for future structural studies. On comparing the two complexes, we observe for the first time the phenomenon of a strand slippage in septins. Such slippage generates a front-back communication mechanism between the G and NC interfaces. This data provides a novel mechanistic framework for the influence of nucleotide binding to the GTPase domain, opening new possibilities for the study of the dynamics of septin filaments. | |||
Crystal structure of a Schistosoma mansoni septin reveals the phenomenon of strand slippage in septins dependent on the nature of the bound nucleotide.,Zeraik AE, Pereira HM, Santos YV, Brandao-Neto J, Spoerner M, Santos MS, Colnago LA, Garratt RC, Araujo AP, Demarco R J Biol Chem. 2014 Jan 24. PMID:24464615<ref>PMID:24464615</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Araujo, A P.U | == References == | ||
[[Category: Brandao-Neto, J | <references/> | ||
[[Category: Demarco, R | __TOC__ | ||
[[Category: Garratt, R C | </StructureSection> | ||
[[Category: Pereira, H M | [[Category: Blood fluke]] | ||
[[Category: Santos, Y V | [[Category: Araujo, A P.U]] | ||
[[Category: Zeraik, A E | [[Category: Brandao-Neto, J]] | ||
[[Category: Demarco, R]] | |||
[[Category: Garratt, R C]] | |||
[[Category: Pereira, H M]] | |||
[[Category: Santos, Y V]] | |||
[[Category: Zeraik, A E]] | |||
[[Category: Cytoskeleton component]] | [[Category: Cytoskeleton component]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Small gtpase]] | [[Category: Small gtpase]] | ||
Revision as of 12:58, 4 January 2015
GTPase domain of Septin 10 from Schistosoma mansoni in complex with GTP
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