4hq8: Difference between revisions

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~~{{STRUCTURE_4hq8| PDB=4hq8 | SCENE= }}~~

==Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state==

===Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state===

~~{{ABSTRACT_PUBMED_24410188}}~~

== Structural highlights ==

<table><tr><td colspan='2'>[[4hq8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HQ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HQ8 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hq8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hq8 RCSB], [http://www.ebi.ac.uk/pdbsum/4hq8 PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Advanced imaging techniques crucially depend on the labels used. In this work, we present the structure-guided design of a fluorescent protein that displays both reversibly photochromic and green-to-red photoconversion behavior. We first designed ffDronpa, a mutant of the photochromic fluorescent protein Dronpa that matures up to three times faster while retaining its interesting photochromic features. Using a combined evolutionary and structure-driven rational design strategy, we developed a green-to-red photoconvertible ffDronpa mutant, called pcDronpa, and explored different optimization strategies that resulted in its improved version, pcDronpa2. This fluorescent probe combines a high brightness with low photobleaching and photoblinking. We herein show that, despite its tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by sequentially imaging a given sample using both super-resolution fluctuation imaging and localization microscopy.

~~==About this Structure==~~

Green-to-Red Photoconvertible Dronpa Mutant for Multimodal Super-resolution Fluorescence Microscopy.,Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, van Meervelt L, Hofkens J, Dedecker P ACS Nano. 2014 Jan 21. PMID:24410188<ref>PMID:24410188</ref>

~~[[4hq8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp~~.~~_sc22 Echinophyllia sp~~. ~~sc22]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4HQ8 OCA].~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

==See Also==

*[[Dronpa|Dronpa]]

== References ==

==~~Reference~~==

~~<ref group="xtra">PMID:024410188</ref>~~<references ~~group="xtra"~~/><~~references~~/>

__TOC__

</StructureSection>

[[Category: Echinophyllia sp. sc22]]

[[Category: Bich, N Nguyen.]]

[[Category: Bich, N Nguyen]]

[[Category: Meervelt, L Van.]]

[[Category: Meervelt, L Van]]

[[Category: Beta-barrel]]

[[Category: Dronpa]]

[[Category: Fluorescent protein]]

[[Category: Green-to-red fluorescent protein]]

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_4hq8|  PDB=4hq8  |  SCENE=  }}
+==Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state==
-===Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state===
+<StructureSection load='4hq8' size='340' side='right' caption='[[4hq8]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
-{{ABSTRACT_PUBMED_24410188}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4hq8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HQ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HQ8 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2z1o|2z1o]], [[2z6y|2z6y]], [[2z6z|2z6z]], [[2vvh|2vvh]], [[4hq9|4hq9]], [[4hqc|4hqc]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hq8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hq8 RCSB], [http://www.ebi.ac.uk/pdbsum/4hq8 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Advanced imaging techniques crucially depend on the labels used. In this work, we present the structure-guided design of a fluorescent protein that displays both reversibly photochromic and green-to-red photoconversion behavior. We first designed ffDronpa, a mutant of the photochromic fluorescent protein Dronpa that matures up to three times faster while retaining its interesting photochromic features. Using a combined evolutionary and structure-driven rational design strategy, we developed a green-to-red photoconvertible ffDronpa mutant, called pcDronpa, and explored different optimization strategies that resulted in its improved version, pcDronpa2. This fluorescent probe combines a high brightness with low photobleaching and photoblinking. We herein show that, despite its tetrameric nature, pcDronpa2 allows for multimodal subdiffraction imaging by sequentially imaging a given sample using both super-resolution fluctuation imaging and localization microscopy.
-==About this Structure==
+Green-to-Red Photoconvertible Dronpa Mutant for Multimodal Super-resolution Fluorescence Microscopy.,Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, van Meervelt L, Hofkens J, Dedecker P ACS Nano. 2014 Jan 21. PMID:24410188<ref>PMID:24410188</ref>
-[[4hq8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HQ8 OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
 ==See Also==
 *[[Dronpa|Dronpa]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:024410188</ref><references group="xtra"/><references/>
+__TOC__
+</StructureSection>
 [[Category: Echinophyllia sp. sc22]]
-[[Category: Bich, N Nguyen.]]
+[[Category: Bich, N Nguyen]]
-[[Category: Meervelt, L Van.]]
+[[Category: Meervelt, L Van]]
 [[Category: Beta-barrel]]
 [[Category: Dronpa]]
 [[Category: Fluorescent protein]]
 [[Category: Green-to-red fluorescent protein]]