3vow: Difference between revisions
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[[ | ==Crystal Structure of the Human APOBEC3C having HIV-1 Vif-binding Interface== | ||
<StructureSection load='3vow' size='340' side='right' caption='[[3vow]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vow]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VOW FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">APOBEC3C, APOBEC1L, PBI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vow OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vow RCSB], [http://www.ebi.ac.uk/pdbsum/3vow PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The human apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3, referred to as A3) proteins are cellular cytidine deaminases that potently restrict retrovirus replication. However, HIV-1 viral infectivity factor (Vif) counteracts the antiviral activity of most A3 proteins by targeting them for proteasomal degradation. To date, the structure of an A3 protein containing a Vif-binding interface has not been solved. Here, we report a high-resolution crystal structure of APOBEC3C and identify the HIV-1 Vif-interaction interface. Extensive structure-guided mutagenesis revealed the role of a shallow cavity composed of hydrophobic or negatively charged residues between the alpha2 and alpha3 helices. This region is distant from the DPD motif (residues 128-130) of APOBEC3G that participates in HIV-1 Vif interaction. These findings provide insight into Vif-A3 interactions and could lead to the development of new pharmacologic anti-HIV-1 compounds. | |||
The APOBEC3C crystal structure and the interface for HIV-1 Vif binding.,Kitamura S, Ode H, Nakashima M, Imahashi M, Naganawa Y, Kurosawa T, Yokomaku Y, Yamane T, Watanabe N, Suzuki A, Sugiura W, Iwatani Y Nat Struct Mol Biol. 2012 Sep 23;19(10):1005-10. doi: 10.1038/nsmb.2378. Epub, 2012 Sep 23. PMID:23001005<ref>PMID:23001005</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Iwatani, Y | [[Category: Iwatani, Y]] | ||
[[Category: Kitamura, S | [[Category: Kitamura, S]] | ||
[[Category: Suzuki, A | [[Category: Suzuki, A]] | ||
[[Category: Watanabe, N | [[Category: Watanabe, N]] | ||
[[Category: Antiviral deffense]] | [[Category: Antiviral deffense]] | ||
[[Category: Apobec3]] | [[Category: Apobec3]] | ||
Revision as of 12:53, 5 January 2015
Crystal Structure of the Human APOBEC3C having HIV-1 Vif-binding Interface
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