1bhn: Difference between revisions

The crystal structures of two isoforms of nucleoside diphosphate kinase, from bovine retina overexpressed in Escherischia coli have been determined, to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and, the fold of the monomer is in agreement with NDP-kinase structures from, other biological sources. Although the polypeptide chains of the two, isoforms differ by only two residues, they crystallize in different space, groups. NBR-A crystallizes in space group P212121 with an entire hexamer, in the asymmetric unit, while NBR-B crystallizes in space group P43212, with a trimer in the asymmetric unit. The highly conserved, nucleotide-binding site observed in other nucleoside diphosphate kinase, structures is also observed here. Both NBR-A and NBR-B were crystallized, in the presence of cGMP. The nucleotide is bound with the base in the anti, conformation. The NBR-A active site contained both cGMP and GDP each bound, at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the, purification process. The NBR-B active site contained only cGMP.

1BHN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with 35G as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Structure known Active Site: . Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BHN OCA].  

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