1h8h: Difference between revisions
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==Overview== | ==Overview== | ||
Analysis of tryptophan mutants of F(1)-ATPase from Escherichia coli [Lobau, et al. (1997) FEBS Lett. 404, 15-18] suggested that nucleotide, concentrations used to grow crystals for the determination of the, structure of bovine F(1)-ATPase [Abrahams et al. (1994) Nature 370, 621-628] would be sufficient to occupy only two catalytic sites, and that, higher concentrations of nucleotide would result in all three sites being, occupied. We have determined the structure of bovine F(1)-ATPase at 2.9 A, resolution with crystals grown in the presence of 5 mM AMPPNP and 5 microM, ADP. Similar to previous structures of bovine F(1)-ATPase determined with, crystals grown in the presence of lower nucleotide concentrations, only, two beta-subunits have bound nucleotide and the third subunit remains, . | Analysis of tryptophan mutants of F(1)-ATPase from Escherichia coli [Lobau, et al. (1997) FEBS Lett. 404, 15-18] suggested that nucleotide, concentrations used to grow crystals for the determination of the, structure of bovine F(1)-ATPase [Abrahams et al. (1994) Nature 370, 621-628] would be sufficient to occupy only two catalytic sites, and that, higher concentrations of nucleotide would result in all three sites being, occupied. We have determined the structure of bovine F(1)-ATPase at 2.9 A, resolution with crystals grown in the presence of 5 mM AMPPNP and 5 microM, ADP. Similar to previous structures of bovine F(1)-ATPase determined with, crystals grown in the presence of lower nucleotide concentrations, only, two beta-subunits have bound nucleotide and the third subunit remains, empty. | ||
==About this Structure== | ==About this Structure== | ||
1H8H is a | 1H8H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG, PO4, ATP, ADP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34] Structure known Active Sites: AC1, AC2, AC3, AC4, AC5, AC6, AC7, AC8, AC9, BC1, BC2, CAT and PLP. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8H OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: f1fo atp synthase]] | [[Category: f1fo atp synthase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:56:27 2007'' | ||
