2j04: Difference between revisions
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'''THE TAU60-TAU91 SUBCOMPLEX OF YEAST TRANSCRIPTION FACTOR IIIC''' | {{Structure | ||
|PDB= 2j04 |SIZE=350|CAPTION= <scene name='initialview01'>2j04</scene>, resolution 3.20Å | |||
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'''THE TAU60-TAU91 SUBCOMPLEX OF YEAST TRANSCRIPTION FACTOR IIIC''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2J04 is a [ | 2J04 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J04 OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly., Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW, Mol Cell. 2006 Oct 20;24(2):221-32. PMID:[http:// | Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly., Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW, Mol Cell. 2006 Oct 20;24(2):221-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17052456 17052456] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
[[Category: transcription factor iiic]] | [[Category: transcription factor iiic]] | ||
[[Category: type 2 | [[Category: type 2 promoter]] | ||
[[Category: yeast rna polymerase iii]] | [[Category: yeast rna polymerase iii]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:35:41 2008'' | ||
Revision as of 15:35, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
THE TAU60-TAU91 SUBCOMPLEX OF YEAST TRANSCRIPTION FACTOR IIIC
Overview
Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA and tauB of transcription factor IIIC (TFIIIC) to the A and B blocks of tRNA gene promoters. The tauB subcomplex consists of subunits tau60, tau91, and tau138. We determined the 3.2 A crystal structure of tau60 bound to a large C-terminal fragment of tau91 (Deltatau91). Deltatau91 protein contains a seven-bladed propeller preceded by an N-terminal extension, whereas tau60 contains a structurally homologous propeller followed by a C-terminal domain with a novel alpha/beta fold. The two propeller domains do not have any detectable DNA binding activity and mediate heterodimer formation that may serve as scaffold for tau138 assembly. We show that the C-terminal tau60 domain interacts with the TATA binding protein (TBP). Recombinant tauB recruits TBP and stimulates TFIIIB-directed transcription on a TATA box containing tRNA gene, implying a combined contribution of tauA and tauB to preinitiation complex formation.
About this Structure
2J04 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly., Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW, Mol Cell. 2006 Oct 20;24(2):221-32. PMID:17052456
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