Sandbox Reserved 951: Difference between revisions

Luciferase is a 62kDa protein. It contains 550 amino acids. The enzyme can be divide into two domains. On the one hand, the major portion, corresponding to the <scene name='60/604470/N_terminal_domain/1'>N-terminal</scene>. On the other hand, the small portion, corresponding to the <scene name='60/604470/C_terminal_domain/1'>C-terminal domain</scene>.Those two domains are separated by a large cleft.<ref name =''first''>PMID:8805533</ref>

The amino acids of C terminal sequence is composed of contiguous residues and form a type of lid upon the N terminal domain.  It contains two β sheets: the <scene name='60/604470/Beta_sheet/2'>first β sheet </scene> is composed of two short antiparallel strands and the  <scene name='60/604470/Beta_sheet_2/1'> second β sheet</scene> is composed of 3 antiparallel strands, mixed with <scene name='60/604470/3_alpha_helices/1'>three α helices</scene>. Those helices are put toward the outside.It is α + β structure.<ref name =''second''>PMID:8805533</ref>

The amino acids of C terminal sequence is composed of non contiguous residues. It falls into three subdomains (<scene name='60/604470/Domain_a/1'>the subdomain A</scene>,<scene name='60/604470/Domain_b/1'>the subdomain B</scene>, and <scene name='60/604470/Domain_c/1'>the subdomain C</scene>).It has an antiparallel <scene name='60/604470/Beta_barrel/3'>β barrel</scene>, two β sheets, which are framed by <scene name='60/604470/Alpha_helices/1'>α helices</scene>. Each of the two β sheets subdomains (A and B)are composed of 8 β strands and 6 helices. The β sheet A has <scene name='60/604470/A_beta_strands/1'>5 parallel and 3 antiparallel β strands</scene>. The β sheet B has <scene name='60/604470/A_beta_strands/1'>6 parallel and 2 antiparallel β strands</scene>. Those β sheets create a groove, closed on one end by the β barrel.<ref name =''third''>PMID:8805533</ref>