2bz0: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
GTP cyclohydrolase II converts GTP to, 2,5-diamino-6-beta-ribosyl-4(3H)-pyrimidinone 5'-phosphate, formate and, pyrophosphate, the first step in riboflavin biosynthesis. The essential, role of riboflavin in metabolism and the absence of GTP cyclohydrolase II, in higher eukaryotes makes it a potential novel selective antimicrobial, drug target. GTP cyclohydrolase II catalyzes a distinctive overall, reaction from GTP cyclohydrolase I; the latter converts GTP to, dihydroneopterin triphosphate, utilized in folate and tetrahydrobiopterin, biosynthesis. The structure of GTP cyclohydrolase II determined at 1.54-A, resolution reveals both a different protein fold to GTP cyclohydrolase I, and distinctive molecular recognition determinants for GTP; although in, both enzymes there is a bound ... | GTP cyclohydrolase II converts GTP to, 2,5-diamino-6-beta-ribosyl-4(3H)-pyrimidinone 5'-phosphate, formate and, pyrophosphate, the first step in riboflavin biosynthesis. The essential, role of riboflavin in metabolism and the absence of GTP cyclohydrolase II, in higher eukaryotes makes it a potential novel selective antimicrobial, drug target. GTP cyclohydrolase II catalyzes a distinctive overall, reaction from GTP cyclohydrolase I; the latter converts GTP to, dihydroneopterin triphosphate, utilized in folate and tetrahydrobiopterin, biosynthesis. The structure of GTP cyclohydrolase II determined at 1.54-A, resolution reveals both a different protein fold to GTP cyclohydrolase I, and distinctive molecular recognition determinants for GTP; although in, both enzymes there is a bound catalytic zinc. The GTP cyclohydrolase, II.GMPCPP complex structure shows Arg(128) interacting with the, alpha-phosphonate, and thus in the case of GTP, Arg(128) is positioned to, act as the nucleophile for pyrophosphate release and formation of the, proposed covalent guanylyl-GTP cyclohydrolase II intermediate. Tyr(105) is, identified as playing a key role in GTP ring opening; it is, hydrogen-bonded to the zinc-activated water molecule, the latter being, positioned for nucleophilic attack on the guanine C-8 atom. Although GTP, cyclohydrolase I and GTP cyclohydrolase II both use a zinc ion for the GTP, ring opening and formate release, different residues are utilized in each, case to catalyze this reaction step. | ||
==About this Structure== | ==About this Structure== | ||
2BZ0 is a | 2BZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN, MG and G2P as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/GTP_cyclohydrolase_II GTP cyclohydrolase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.25 3.5.4.25] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BZ0 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 31: | Line 31: | ||
[[Category: riboflavin biosynthesis]] | [[Category: riboflavin biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:01:55 2007'' | ||
