2nrn: Difference between revisions
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[[Image:2nrn.gif|left|200px]] | [[Image:2nrn.gif|left|200px]] | ||
'''Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant''' | {{Structure | ||
|PDB= 2nrn |SIZE=350|CAPTION= <scene name='initialview01'>2nrn</scene>, resolution 1.40Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | |||
|ACTIVITY= | |||
|GENE= GCN4, AAS3, ARG9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2NRN is a [ | 2NRN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NRN OCA]. | ||
==Reference== | ==Reference== | ||
Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant., Deng Y, Zheng Q, Liu J, Cheng CS, Kallenbach NR, Lu M, Protein Sci. 2007 Feb;16(2):323-8. Epub 2006 Dec 22. PMID:[http:// | Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant., Deng Y, Zheng Q, Liu J, Cheng CS, Kallenbach NR, Lu M, Protein Sci. 2007 Feb;16(2):323-8. Epub 2006 Dec 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17189475 17189475] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Lu, M.]] | [[Category: Lu, M.]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: coiled | [[Category: coiled coil]] | ||
[[Category: protein design]] | [[Category: protein design]] | ||
[[Category: protein structure]] | [[Category: protein structure]] | ||
[[Category: tetramer]] | [[Category: tetramer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:50:09 2008'' | ||
Revision as of 15:50, 20 March 2008
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| 2nrn, resolution 1.40Å | |||||||||||||
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| Ligands: | PO4 | ||||||||||||
| Gene: | GCN4, AAS3, ARG9 (Saccharomyces cerevisiae) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant
Overview
The hydrophobic core of the GCN4 leucine-zipper dimerization domain is formed by a parallel helical association between nonpolar side chains at the a and d positions of the heptad repeat. Here we report a self-assembling coiled-coil array formed by the GCN4-pAe peptide that differs from the wild-type GCN4 leucine zipper by alanine substitutions at three charged e positions. GCN4-pAe is incompletely folded in normal solution conditions yet self-assembles into an antiparallel tetraplex in crystals by formation of unanticipated hydrophobic seams linking the last two heptads of two parallel double-stranded coiled coils. The GCN4-pAe tetramers in the lattice associate laterally through the identical interactions to those in the intramolecular dimer-dimer interface. The van der Waals packing interaction in the solid state controls extended supramolecular assembly of the protein, providing an unusual atomic scale view of a mesostructure.
About this Structure
2NRN is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant., Deng Y, Zheng Q, Liu J, Cheng CS, Kallenbach NR, Lu M, Protein Sci. 2007 Feb;16(2):323-8. Epub 2006 Dec 22. PMID:17189475
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