2nu5: Difference between revisions
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[[Image:2nu5.jpg|left|200px]] | [[Image:2nu5.jpg|left|200px]] | ||
'''Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine''' | {{Structure | ||
|PDB= 2nu5 |SIZE=350|CAPTION= <scene name='initialview01'>2nu5</scene>, resolution 1.564Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2NU5 is a [ | 2NU5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Griffithsia_sp._q66d336 Griffithsia sp. q66d336]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NU5 OCA]. | ||
==Reference== | ==Reference== | ||
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:[http:// | Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17567736 17567736] | ||
[[Category: Griffithsia sp. q66d336]] | [[Category: Griffithsia sp. q66d336]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: antiviral protein]] | [[Category: antiviral protein]] | ||
[[Category: griffithsin; lectins; domain swapping; mannose binding; hiv; | [[Category: griffithsin; lectins; domain swapping; mannose binding; hiv; sar]] | ||
[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:51:13 2008'' | ||
Revision as of 15:51, 20 March 2008
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| 2nu5, resolution 1.564Å | |||||||||||||
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| Ligands: | NAG and SO4 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
About this Structure
2NU5 is a Single protein structure of sequence from Griffithsia sp. q66d336. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736
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