2juk

guanidino neomycin B recognition of an HIV-1 RNA helix

spinqualitylabelsall models 2juk, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2juk is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: G0B Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed Aminoglycoside antibiotics are small-molecule drugs that bind RNA. The affinity and specificity of aminoglycoside binding to RNA can be increased through chemical modification, such as guanidinylation. Here, we report the binding of guanidinoneomycin B (GNB) to an RNA helix from the HIV-1 frameshift site. The binding of GNB increases the melting temperature (T(m)) of the frameshift-site RNA by at least 10 degrees C, to a point at which a melting transition is not even observed in 2 M urea. A structure of the complex was obtained by using multidimensional heteronuclear NMR spectroscopic methods. We also used a novel paramagnetic-probe assay to identify the site of GNB binding to the surface of the RNA. GNB makes major-groove contacts to two sets of Watson-Crick bases and is in van der Waals contact with a highly structured ACAA tetraloop. Rings I and II of GNB fit into the major groove and form the binding interface with the RNA, whereas rings III and IV are exposed to the solvent and disordered. The binding of GNB causes a broadening of the major groove across the binding site. Guanidinoneomycin B recognition of an HIV-1 RNA helix.,Staple DW, Venditti V, Niccolai N, Elson-Schwab L, Tor Y, Butcher SE Chembiochem. 2008 Jan 4;9(1):93-102. PMID:18058789 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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