4aod: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[ | ==Biomphalaria glabrata Acetylcholine-binding protein type 1 (BgAChBP1)== | ||
<StructureSection load='4aod' size='340' side='right' caption='[[4aod]], [[Resolution|resolution]] 6.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4aod]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Biomphalaria_glabrata Biomphalaria glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AOD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AOD FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aoe|4aoe]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aod RCSB], [http://www.ebi.ac.uk/pdbsum/4aod PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nicotinic acetylcholine receptors (nAChR) play important neurophysiological roles and are of considerable medical relevance. They have been studied extensively, greatly facilitated by the gastropod acetylcholine-binding proteins (AChBP) which represent soluble structural and functional homologues of the ligand-binding domain of nAChR. All these proteins are ring-like pentamers. Here we report that AChBP exists in the hemolymph of the planorbid snail Biomphalaria glabrata (vector of the schistosomiasis parasite) as a regular pentagonal dodecahedron, 22 nm in diameter (12 pentamers, 60 active sites). We sequenced and recombinantly expressed two approximately 25 kDa polypeptides (BgAChBP1 and BgAChBP2) with a specific active site, N-glycan site and disulfide bridge variation. We also provide the exon/intron structures. Recombinant BgAChBP1 formed pentamers and dodecahedra, recombinant BgAChBP2 formed pentamers and probably disulfide-bridged di-pentamers, but not dodecahedra. Three-dimensional electron cryo-microscopy (3D-EM) yielded a 3D reconstruction of the dodecahedron with a resolution of 6 A. Homology models of the pentamers docked to the 6 A structure revealed opportunities for chemical bonding at the inter-pentamer interfaces. Definition of the ligand-binding pocket and the gating C-loop in the 6 A structure suggests that 3D-EM might lead to the identification of functional states in the BgAChBP dodecahedron. | |||
Acetylcholine-Binding Protein in the Hemolymph of the Planorbid Snail Biomphalaria glabrata Is a Pentagonal Dodecahedron (60 Subunits).,Saur M, Moeller V, Kapetanopoulos K, Braukmann S, Gebauer W, Tenzer S, Markl J PLoS One. 2012;7(8):e43685. Epub 2012 Aug 20. PMID:22916297<ref>PMID:22916297</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[Acetylcholine binding protein|Acetylcholine binding protein]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Biomphalaria glabrata]] | [[Category: Biomphalaria glabrata]] | ||
[[Category: Braukmann, S | [[Category: Braukmann, S]] | ||
[[Category: Gebauer, W | [[Category: Gebauer, W]] | ||
[[Category: Kapetanopoulos, K | [[Category: Kapetanopoulos, K]] | ||
[[Category: Markl, J | [[Category: Markl, J]] | ||
[[Category: Moeller, V | [[Category: Moeller, V]] | ||
[[Category: Saur, M | [[Category: Saur, M]] | ||
[[Category: Tenzer, S | [[Category: Tenzer, S]] | ||
[[Category: Acetylcholine]] | [[Category: Acetylcholine]] | ||
[[Category: Acetylcholine binding protein]] | [[Category: Acetylcholine binding protein]] | ||
