2wfh

THE HUMAN SLIT 2 DIMERIZATION DOMAIN D4

spinqualitylabelsall models 2wfh, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2wfh is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SO4 Related: 2v9t, 2v9s, 2v70 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Slit proteins are secreted ligands that interact with the Roundabout (Robo) receptors to provide important guidance cues in neuronal and vascular development. Slit-Robo signalling is mediated by an interaction between the second Slit domain and the first Robo domain, as well as being dependent on heparan sulphate. In an effort to understand the role of the other Slit domains in signalling, we determined the crystal structure of the fourth Slit2 domain (D4) and examined the effects of various Slit2 constructs on chick retinal ganglion cell axons. Slit2 D4 forms a homodimer using the conserved residues on its concave face, and can also bind to heparan sulphate. We observed that Slit2 D4 frequently results in growth cones with collapsed lamellipodia and that this effect can be inhibited by exogenously added heparan sulphate. Our results show that Slit2 D4-heparan sulphate binding contributes to a Slit-Robo signalling mechanism more intricate than previously thought. Structure and functional relevance of the Slit2 homodimerization domain.,Seiradake E, von Philipsborn AC, Henry M, Fritz M, Lortat-Jacob H, Jamin M, Hemrika W, Bastmeyer M, Cusack S, McCarthy AA EMBO Rep. 2009 Jul;10(7):736-41. Epub 2009 Jun 5. PMID:19498462 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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