2oiu: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2oiu.gif|left|200px]] | [[Image:2oiu.gif|left|200px]] | ||
'''L1 Ribozyme Ligase circular adduct''' | {{Structure | ||
|PDB= 2oiu |SIZE=350|CAPTION= <scene name='initialview01'>2oiu</scene>, resolution 2.60Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''L1 Ribozyme Ligase circular adduct''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2OIU is a [ | 2OIU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIU OCA]. | ||
==Reference== | ==Reference== | ||
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http:// | The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17363667 17363667] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Robertson, M P]] | [[Category: Robertson, M P]] | ||
| Line 21: | Line 30: | ||
[[Category: rna]] | [[Category: rna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:00:15 2008'' | ||
Revision as of 16:00, 20 March 2008
| |||||||||||||
| 2oiu, resolution 2.60Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | MG | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
L1 Ribozyme Ligase circular adduct
Overview
Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.
About this Structure
2OIU is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667
Page seeded by OCA on Thu Mar 20 18:00:15 2008
